Metabolic fate of ochratoxin A as a coffee contaminant in a dynamic simulator of the human colon
| Autor: | Minale Ouethrani, Ellen Verbeke, Sylvie Rabot, Valérie Camel, Maryline Carvalho, Tom Van de Wiele, Aurélia Bruneau |
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| Přispěvatelé: | Ingénierie Procédés Aliments (GENIAL), Institut National de la Recherche Agronomique (INRA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-AgroParisTech-Conservatoire National des Arts et Métiers [CNAM] (CNAM), Lab Microbial Ecol & Technol LabMET, Universiteit Gent = Ghent University [Belgium] (UGENT), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, international interdisciplinary Ph.D. programme 'Frontiers in Life Sciences', Hubert Curien Partnership (PHC) TOURNESOL FL (France and Belgium, Flemish community) programme [20370QC], Ghent University [Belgium] (UGENT), Institut National de la Recherche Agronomique (INRA)-AgroParisTech-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Ochratoxin A
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences Colon Coffea Food Contamination Gut flora Models Biological Coffee Analytical Chemistry Microbiology RATS 03 medical and health sciences chemistry.chemical_compound GRADIENT GEL-ELECTROPHORESIS Humans VITRO DIGESTION MODEL Food science Mycotoxin Ochratoxin 030304 developmental biology 2. Zero hunger Human feces 0303 health sciences SHIME biology 030306 microbiology Human intestinal microbiota Microbiota 16S RIBOSOMAL-RNA GUT MICROBIOTA food and beverages INTESTINAL MICROBIAL ECOSYSTEM General Medicine IN-VITRO 15. Life on land DEGRADATION biology.organism_classification Ochratoxins Lactobacillus reuteri chemistry 13. Climate action BACTERIA Biodegradation Digestion HUMAN FECES Food Science |
| Zdroj: | Food Chemistry Food Chemistry, Elsevier, 2013, 141 (4), pp.3291-3300. ⟨10.1016/j.foodchem.2013.05.157⟩ |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2013.05.157⟩ |
| Popis: | Ochratoxin A (OTA) is a mycotoxin frequently encountered in coffee. The relevance of this contaminant in the colon upon digestion necessitates a study on its interaction with colon microbiota. Here, the fate of OTA during colon digestion was investigated using a dynamic simulator of the human gut. The influence of coffee as a food matrix was taken into account, as it may affect the colonic microbial ecosystem and, consequently, the fate of OTA. Biodegradation was followed by measuring OTA concentration over time, and by screening for several possible metabolites, using LC-ESI-MS and HRMS. The descending colon was found to be the main site of OTA biodegradation. Two metabolites, ochratoxin a and ochratoxin B, were identified, suggesting that biodegradation by gut microbiota is beneficial for the host, as they are considered less toxic than OTA. The extent of biodegradation was reduced in the presence of the coffee matrix, possibly due to competition for available carbon sources. Effects of OTA and the coffee matrix on the microbial ecosystem were contrasting. While OTA caused a specific, but lasting loss, of the beneficial species Lactobacillus reuteri, coffee temporarily altered the fermentation pattern towards lower ammonia and higher acetate and propionate production, likely due to its dietary fibre content. (C) 2013 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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