Utility of Panfungal PCR in the diagnosis of invasive fungal infections in febrile neutropenia

Autor:	Pushpa Kumari, Sujay Srinivas, Dipendra Kumar Gupta
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	medicine.medical_specialty education.field_of_study invasive fungal infections business.industry Population Gold standard (test) Neutropenia medicine.disease Highly sensitive Diagnostic modalities Internal medicine medicine neutropenia Medicine Histopathology In patient education business panfungal pcr Febrile neutropenia
Zdroj:	Journal of Family Medicine and Primary Care, Vol 10, Iss 7, Pp 2533-2540 (2021)
ISSN:	2249-4863
Popis:	Background: The prevalence of invasive fungal infections (IFIs) is increasing due to the increasing population of immunocompromised patients. Fungal culture is the gold standard for diagnosis but not sensitive and the turnaround time is long. Samples for histopathology are difficult to obtain because of profound cytopenias. We conducted this study with the aim to evaluate panfungal PCR for the diagnosis of IFIs in patients of febrile neutropenia. Methods: This was a single-centre, cross-sectional observational study. Patients of febrile neutropenia suspected of having IFI were included in the study. Panfungal PCR was performed on the blood of included patients along with other investigations for diagnosis of IFI. The sensitivity, specificity, positive predictive value, and negative predictive value of panfungal PCR were calculated using EORTC/MSG 2008 criteria as the gold standard. Results: Fifty patients of febrile neutropenia were included in the study, of which 52% were diagnosed positive by panfungal PCR assay. The sensitivity, specificity, positive predictive value, and negative predictive value of panfungal PCR assay was found to be 82.76%, 90.48%, 92.31% and 79.17% respectively. Conclusion: Panfungal PCR is a promising and highly sensitive diagnostic test for screening at-risk patients suspected to have IFIs. The use of panfungal PCR assay in combination with other diagnostic modalities and clinical judgment can be very helpful in the early diagnosis of IFI.
Databáze:	OpenAIRE
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