Effects of Aqueous and Methanolic Extracts of Stem Bark of Alstonia boonei De Wild. (Apocynaceae) on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Wistar Rats
| Autor: | Carine Flore Adjouzem, Eric Gonzal Tsafack, Albert Donatien Atsamo, Ateufack Gilbert, Marius Mbiantcha, Stephanie Flore Djuichou Nguemnang, William Yousseu Nana, Vanessa Matah Marthe |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Article Subject
ved/biology.organism_classification_rank.species Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Other systems of medicine 0302 clinical medicine medicine Colitis Alstonia boonei 030304 developmental biology 0303 health sciences Traditional medicine Apocynaceae biology ved/biology Glutathione biology.organism_classification medicine.disease Malondialdehyde Complementary and alternative medicine chemistry Phytochemical Catalase biology.protein 030211 gastroenterology & hepatology RZ201-999 |
| Zdroj: | Evidence-Based Complementary and Alternative Medicine, Vol 2020 (2020) |
| ISSN: | 1741-4288 |
| Popis: | Among the most exploited species in Cameroon, Alstonia boonei is widely used in African medicine for the relief of several pathologies including gastrointestinal disorders. This study was conducted in order to assess the effects of aqueous and methanol stem-bark extracts of Alstonia boonei on DSS- (dextran sodium sulfate-) induced intestinal colitis and to determine its antioxidant potential. The classes of secondary metabolites present in these extracts were determined by chemical screening. The production of TNF-α, IL-6, IL-1β, and PGE2 was performed by in vitro ELISA analysis. Anticolitis effects were determined using an in vivo model of ulcerative colitis induced by DSS. The colitis was induced with a double dose of DSS (3% and 1%), and the aqueous and methanol extracts were administered orally from the 6th day after commencement of induction. The phytochemical screening revealed the presence of six classes of secondary metabolites in these crude extracts: tannins, saponins, alkaloids, steroids, flavonoids, and phenols. Methanol and aqueous extracts of Alstonia boonei significantly (P<0.001) inhibited TNF-α, IL-6, IL-1β, and PGE2 production stimulated by LPS. Both extracts at all doses significantly reduced (P<0.01, P<0.001) the signs of DSS-induced colitis in the Wistar rats by decreasing inflammation and chronic colon damage. In addition, the extracts significantly (P<0.001) reduced malondialdehyde and nitric oxide levels in the colon and significantly (P<0.01) increased superoxide dismutase and catalase and reduced glutathione (P<0.05). Both extracts showed greater activity than the reference substance (prednisolone 4 mg/kg) used in this study. This study has demonstrated that aqueous and methanol extracts of Alstonia boonei stem bark have healing properties against colitis experimentally induced by DSS in rats. |
| Databáze: | OpenAIRE |
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