Efficient strategies for TALEN-mediated genome editing in mammalian cell lines

Autor: Fayza Daboussi, Séverine Thomas, Alexandre Juillerat, Jean-Pierre Cabaniols, Céline Lebuhotel, Annabelle Gariboldi, Julien Valton, Fabien Delacôte, Sebastien Paris, Adeline Jacquet, Sandra Rolland, Domique Alain Blanchard, Marianne Duhamel, Gil Letort, Sandra Moriceau, Raffy Demirdjian, Aymeric Duclert, Roman Galetto, Philippe Duchateau, Claudia Bertonati
Rok vydání: 2014
Předmět:
Zdroj: Methods. 69:151-170
ISSN: 1046-2023
Popis: TALEN is one of the most widely used tools in the field of genome editing. It enables gene integration and gene inactivation in a highly efficient and specific fashion. Although very attractive, the apparent simplicity and high success rate of TALEN could be misleading for novices in the field of gene editing. Depending on the application, specific TALEN designs, activity assessments and screening strategies need to be adopted. Here we report different methods to efficiently perform TALEN-mediated gene integration and inactivation in different mammalian cell systems including induced pluripotent stem cells and delineate experimental examples associated with these approaches.
Databáze: OpenAIRE