Preparation of alginate–chitosan–cyclodextrin micro- and nanoparticles loaded with anti-tuberculosis compounds
| Autor: | Fatma Aksakal, Albert Ivancic, Fliur Macaev, Gheorghe Duca, Veaceslav Boldescu, Serghei Pogrebnoi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
quaternary system isoniazid Materials science medicine.drug_class 030106 microbiology General Physics and Astronomy 02 engineering and technology lcsh:Chemical technology Antimycobacterial lcsh:Technology Full Research Paper sodium alginate Chitosan 03 medical and health sciences chemistry.chemical_compound Isoconazole density functional theory (DFT) medicine Organic chemistry Nanotechnology lcsh:TP1-1185 General Materials Science Electrical and Electronic Engineering lcsh:Science chemistry.chemical_classification biology Cyclodextrin lcsh:T INHA Isoniazid Active site molecular docking 021001 nanoscience & nanotechnology Combinatorial chemistry lcsh:QC1-999 Nanoscience chemistry isoconazole Docking (molecular) β-cyclodextrin biology.protein lcsh:Q chitosan 0210 nano-technology lcsh:Physics medicine.drug |
| Zdroj: | Beilstein Journal of Nanotechnology Beilstein Journal of Nanotechnology, Vol 7, Iss 1, Pp 1208-1218 (2016) |
| ISSN: | 2190-4286 |
| Popis: | This paper describes the synthesis and application of alginate–chitosan–cyclodextrin micro- and nanoparticulate systems loaded with isoniazid (INH) and isoconazole nitrate (ISN) as antimycobacterial compounds. Preparation and morphology of the obtained particles, as well as antimycobacterial activity data of the obtained systems are presented. Docking of isoconazole into the active site of enoyl–acyl carrier protein reductase (InhA) of Mycobacetrium tuberculosis was carried out in order to predict the binding affinity and non-covalent interactions stabilizing the InhA–isoconazole complex. To assess these interactions, frontier molecular orbital calculations were performed for the active site of InhA and isoconazole obtained from docking. Isoconazole was predicted to be an active inhibitor of InhA with the analysis of the molecular docking and electron density distribution. It has been detected that alginate–chitosan–cyclodextrin microparticulate systems loaded with INH and ISN are as effective as pure INH applied in higher dosages. |
| Databáze: | OpenAIRE |
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