Does the mouse tail vein injection method provide a good model of lung cancer?
| Autor: | Rhonda J. Rosengren, Mhairi Nimick, Nensi Shrestha, Orleans N. K. Martey, John C. Ashton, Abigail R. Bland, Zabeen Lateef |
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| Rok vydání: | 2019 |
| Předmět: |
tail vein
Pathology medicine.medical_specialty Lung Neoplasms mouse model General Biochemistry Genetics and Molecular Biology Carcinoma Lewis Lung Mice Medicine Animals Humans General Pharmacology Toxicology and Pharmaceutics Lung cancer Lung General Immunology and Microbiology business.industry human cells Cancer Lewis lung carcinoma General Medicine Articles respiratory system medicine.disease respiratory tract diseases lung cancer Disease Models Animal medicine.anatomical_structure Drug development Cell culture A549 Cells Cancer cell Immunohistochemistry business Neoplasm Transplantation Research Article |
| Zdroj: | F1000Research |
| ISSN: | 2046-1402 |
| Popis: | Lung cancer drug development requires screening in animal models. We aimed to develop orthotopic models of human non-small lung cancer using A549 and H3122 cells delivered by tail vein injection. This procedure has been used previously for a mouse lung cancer (Lewis lung carcinoma) and as a model of human breast cancer metastasis to lung. We report that the procedure led to poor animal condition 7-8 weeks after injection, and produced lesions in the lungs visible at necropsy but we were unable identify individual cancer cells using immunohistochemistry. We conclude that if this method is to produce a model that can be used in drug experiments, improvements are required for cancer cell detection post mortem, such as by using of a fluorescently tagged human lung cancer cell line. |
| Databáze: | OpenAIRE |
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