A selective insecticidal protein fromPseudomonas mosseliifor corn rootworm control
| Autor: | Albert L. Lu, Nasser Yalpani, Gusui Wu, Genhai Zhu, Scott Diehn, Claudia Perez Ortega, Mark J. McDonald, Jun-Zhi Wei, Jessica O'rear, Park Young-Jun, Mark E. Nelson, Lisa Procyk, Gary A. Sandahl, Jian-Zhou Zhao, Lu Tian Liu, Barbara Rosen, Ute Schellenberger, Virginia C. Crane, Adane Kassa, Weiping Xie |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Diabrotica Bacillus thuringiensis western corn rootworm Plant Science Zea mays 01 natural sciences Pseudomonas mosselii 03 medical and health sciences mode of action Pseudomonas Botany Animals Pest Control Biological Research Articles Diabrotica undecimpunctata Genetically modified maize biology fungi food and beverages Plants Genetically Modified insecticidal protein biology.organism_classification Diabrotica speciosa 010602 entomology 030104 developmental biology Western corn rootworm Spotted lady beetle Agronomy and Crop Science Research Article Biotechnology |
| Zdroj: | Plant Biotechnology Journal |
| ISSN: | 1467-7644 |
| DOI: | 10.1111/pbi.12806 |
| Popis: | Summary The coleopteran insect western corn rootworm (WCR, Diabrotica virgifera virgifera) is an economically important pest in North America and Europe. Transgenic corn plants producing Bacillus thuringiensis (Bt) insecticidal proteins have been useful against this devastating pest, but evolution of resistance has reduced their efficacy. Here, we report the discovery of a novel insecticidal protein, PIP‐47Aa, from an isolate of Pseudomonas mosselii. PIP‐47Aa sequence shows no shared motifs, domains or signatures with other known proteins. Recombinant PIP‐47Aa kills WCR, two other corn rootworm pests (Diabrotica barberi and Diabrotica undecimpunctata howardi) and two other beetle species (Diabrotica speciosa and Phyllotreta cruciferae), but it was not toxic to the spotted lady beetle (Coleomegilla maculata) or seven species of Lepidoptera and Hemiptera. Transgenic corn plants expressing PIP‐47Aa show significant protection from root damage by WCR. PIP‐47Aa kills a WCR strain resistant to mCry3A and does not share rootworm midgut binding sites with mCry3A or AfIP‐1A/1B from Alcaligenes that acts like Cry34Ab1/Cry35Ab1. Our results indicate that PIP‐47Aa is a novel insecticidal protein for controlling the corn rootworm pests. |
| Databáze: | OpenAIRE |
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