Vagococcus salmoninarum II-qPCR, tropism and egg-associated transmission
| Autor: | Sara Erickson, Jorge Buening, Thomas P. Loch, Eric Leis, Ryan Katona, Eric Kleman, Jennifer Bailey, Rebekah McCann, Isaac Standish, Ellen Lark, Kenneth Phillips, Carey Edwards, Corey Puzach |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Trout Veterinary (miscellaneous) Enterococcaceae Zoology Broodstock Aquaculture Aquatic Science Polymerase Chain Reaction 03 medical and health sciences Fish Diseases Wisconsin medicine Prevalence Animals Vagococcus salmoninarum Epizootic Tropism Gram-Positive Bacterial Infections Salvelinus Ovum biology 04 agricultural and veterinary sciences biology.organism_classification medicine.disease Viral Tropism 030104 developmental biology 040102 fisheries Fish hatchery Tissue tropism 0401 agriculture forestry and fisheries Female |
| Zdroj: | Journal of fish diseasesREFERENCES. 43(3) |
| ISSN: | 1365-2761 |
| Popis: | Vagococcus salmoninarum was identified as the causative agent of a chronic epizootic in broodstock "coaster" brook trout (Salvelinus fontinalis) at the Iron River National Fish Hatchery. The epizootic spanned more than a year, was unresponsive to multiple florfenicol treatments, and resulted in >50% mortality of the affected fish. The decision was made to cull the remaining fish during spawning, which presented an opportunity to more thoroughly examine V. salmoninarum sampling methods, organ tropism and vertical transmission. A newly developed qPCR targeting the pheS gene was used in concert with bacterial culture to show that V. salmoninarum indeed disproportionately affects females and has a tropism for female reproductive tissues. The study demonstrates that some female reproductive tissues (e.g. ovarian fluid, unfertilized eggs) are also an effective option for non-lethal detection. Despite the widespread presence of V. salmoninarum in ovarian fluid and on egg surfaces, we found no evidence of intra-ova transmission. |
| Databáze: | OpenAIRE |
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