Increase of poly(ADP-ribose) polymerase mRNA levels during TPA-induced differentiation of human lymphocytes
| Autor: | Hisanori Suzuki, Giorgio Gandini, Franca Gerosa, Masanao Miwa, Alessandra Carecreri de Prati, Marta Menegazzi, Marina Tommasi |
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| Rok vydání: | 1992 |
| Předmět: |
Lymphocyte
Poly ADP ribose polymerase Cellular differentiation Biophysics Fluorescent Antibody Technique Stimulation Biology Biochemistry Peripheral blood mononuclear cell Differentiation Poly(ADP-ribose) polymerase Lymphocyte TPA Antigen Structural Biology Gene expression Genetics medicine Humans Lymphocytes RNA Messenger Molecular Biology Messenger RNA Poly(ADP-ribose) polymerase Cell Differentiation Cell Biology Blotting Northern Flow Cytometry Molecular biology medicine.anatomical_structure Tetradecanoylphorbol Acetate Poly(ADP-ribose) Polymerases Cell Division |
| Zdroj: | FEBS Letters. 297:59-62 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(92)80327-d |
| Popis: | The non-mitogenic stimulation of human peripheral blood mononuclear cells (PBMC) with low concentrations of the phorbol ester 12-O-tetradecanoylphorbol 13-acatate (TPA) caused a progressive increase in the percent fraction of the cells that were positive for the early activating antigen CD69. At the same time, it caused a progressive increase in the steady-state levels of poly(ADP-ribose) polymerase (pADPRP) transcripts. A further increase in TPA concentration, while inducing the maximal expression of the levels of CD69 activating surface antigen, both in the presence or in the absence of proliferative activity, did not evoke any additional hightening of pADPRP mRNA levels. Time course of PBMC stimulation with a non-mitogenic dose or TPA showed an early increase in the accumulation of pADPRP mRNA, which changed at 8-16 h. and remained high for several days thereafter. On the basis of these data, we suggest flat the increase in pADPRP mRNA may be associated with the commitment of human lymphocytes from a quiescent (G0) to an activated (G1) state. |
| Databáze: | OpenAIRE |
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