Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation
| Autor: | Ana Rita A. Nogueira, S. R. Bianchi, Clarice D.B. Amaral, Caroline Santos Silva |
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| Přispěvatelé: | SILMARA ROSSANA BIANCHI, CNPS, Clarice Dias Britto do Amaral, UFPR, Caroline Santos da Silva, UFSCar, ANA RITA DE ARAUJO NOGUEIRA, CPPSE. |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
endocrine system
Acid digestion Chromatography Formic acid 010401 analytical chemistry Cattle fertility chemistry.chemical_element General Chemistry 010501 environmental sciences 01 natural sciences Bovine semen 0104 chemical sciences Nebulizer chemistry.chemical_compound chemistry Solubilization Sample preparation selenium Inductively coupled plasma mass spectrometry Selenium 0105 earth and related environmental sciences |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| Popis: | Determination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+ , 77Se+ , 78Se+ and 82Se+ without using CRI and 82Se+ with CRI. Made available in DSpace on 2021-09-21T18:00:55Z (GMT). No. of bitstreams: 1 Determination-of-selenium-in-bovine-semen-2017.pdf: 361824 bytes, checksum: b906bdbe3846b974b96522244d78e39d (MD5) Previous issue date: 2017 |
| Databáze: | OpenAIRE |
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