Control of cell growth by c-Myc in the absence of cell division
| Autor: | Alexander Pajic, Dirk Eick, Martin S. Staege, Marino Schuhmacher, Franz Kohlhuber, Georg W. Bornkamm, Axel Polack, Ulrich H. Weidle |
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| Rok vydání: | 1999 |
| Předmět: |
Agricultural and Biological Sciences(all)
Cell division Biochemistry Genetics and Molecular Biology(all) Cell growth Chromosomal translocation Biology medicine.disease Molecular biology General Biochemistry Genetics and Molecular Biology Culture Media Lymphoma Proto-Oncogene Proteins c-myc Cell culture medicine Protein biosynthesis Humans General Agricultural and Biological Sciences Gene Transcription factor Cell Division Cell Line Transformed Cell Size |
| Zdroj: | Current Biology. 9:1255-1258 |
| ISSN: | 0960-9822 |
| Popis: | The c-Myc protein (Myc) is a transcription factor, and deregulated expression of the c-myc gene (myc) is frequently found in tumours. In Burkitt's lymphoma (BL), myc is transcriptionally activated by chromosomal translocation. We have used a B-cell line called P493-6 that carries a conditional myc allele to elucidate the role of Myc in the proliferation of BL cells. Regulation of proliferation involves the coordination of cell growth (accumulation of cell mass) and cell division [1–3]. Here, we show that division of P493-6 cells was strictly dependent on the expression of the conditional myc allele and the presence of foetal calf serum (FCS). More importantly, cell growth was regulated by Myc without FCS: Myc alone induced an increase in cell size and positively regulated protein synthesis. An increase in protein synthesis is thought to be one of the causes of cell mass increase. Furthermore, Myc stimulated metabolic activities, as indicated by the acidification of culture medium and the activation of mitochondrial enzymes. Our results confirm the model that Myc is involved in the regulation of cell growth [4] and provide, for the first time, direct evidence that Myc induces cell growth, that is, an increase in cell size, uncoupled from cell division. |
| Databáze: | OpenAIRE |
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