Sonochemiluminescence Using Apertureless USB Piezoelectric Ultrasonic Transducer and Its Applications for the Detection of Hydrogen Peroxide, Glucose, and Glucose Oxidase Activity
| Autor: | Dmytro Snizhko, Aimin Wang, Abubakar Abdussalam, Wei Zhang, Fangxin Du, Chengda Meng, Guobao Xu |
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| Rok vydání: | 2021 |
| Předmět: |
Detection limit
biology business.industry Transducers Substrate (chemistry) Biosensing Techniques Hydrogen Peroxide Analytical Chemistry Luminol Sonochemistry Glucose Oxidase Glucose oxidase activity chemistry.chemical_compound Glucose chemistry Linear range Limit of Detection biology.protein Optoelectronics Ultrasonics Glucose oxidase business Hydrogen peroxide |
| Zdroj: | Analytical Chemistry. 93:14934-14939 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | The mesh-type USB piezoelectric ultrasonic transducer (USB-PUT) used in household humidifiers and inhalation therapy devices is very cheap, small, and energy saving. It holds great promise for sonochemistry. However, the microtapered apertures in the center of the stainless steel substrate of mesh-type USB-PUT can lead to rapid atomization of solution, leakage of solutions containing surfactants and organic solvent through the apertures, and high background emission. Herein, we design a new type of USB-PUT by replacing the meshed stainless steel substrate with an apertureless stainless steel substrate. We have found that this apertureless USB-PUT can not only induce intense sonochemiluminescence (SCL) but can also enable sensitive luminol SCL detection of hydrogen peroxide which is practically impossible using mesh-type PUT because of the strong background SCL emission. By using this apertureless device to induce SCL and using smart phone as a detector, a visual hydrogen peroxide SCL detection method with a linear range of 0.5-50 μM and a detection limit of 0.32 μM is established. Moreover, the device can achieve the detection of glucose oxidase (GOD) activity and glucose by enzymatic conversion of glucose to hydrogen peroxide. The linear range of GOD detection is 1-200U/L with a detection limit of 0.86 U/L. The linear range of glucose detection is 0.5-70 μM with a detection limit of 0.43 μM. The cheap (a few dollars) and user-friendly apertureless USB-PUT is promising for sonochemistry applications and chemical education. |
| Databáze: | OpenAIRE |
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