The adjuvant-free immunoprotection of recombinant filarial protein Abundant Larval Transcript-2 (ALT-2) in Mastomys coucha and the immunoprophylactic importance of its putative signal sequence
| Autor: | Sam Sharmila, Maryada Venkata Rami Reddy, Pote Kiran, Immanuel Christiana, Perumal Kaliraj |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Signal peptide Mastomys coucha medicine.medical_treatment Immunology Antibodies Helminth Gene Expression Protein Sorting Signals Lymphocyte Activation Brugia malayi law.invention Filariasis Immune system law Immunity parasitic diseases medicine Animals Immunity Cellular Vaccines Synthetic biology General Medicine biology.organism_classification medicine.disease Virology Recombinant Proteins Disease Models Animal Infectious Diseases Antigens Helminth Immunoglobulin G Recombinant DNA Cytokines Parasitology Murinae Adjuvant Spleen |
| Zdroj: | Experimental Parasitology. 129:247-253 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2011.08.005 |
| Popis: | The filarial protein Abundant Larval Transcript-2 (ALT-2) of the filarial parasite Brugia malayi has been shown to produce 74% worm clearance when administered with an adjuvant. In the present study, we show that it not only induces humoral and cell-mediated immunity, but also protection up to 71% in Mastomys coucha, a permissive animal model for filariasis, even without adjuvant. This unique feature of ALT-2 protein is highly restricted to its 21 amino acid N-terminal signal sequence, the absence of which resulted in poor immune response as well as immunoprotection (49%). Moreover, ALT-2 is likely to exert immunoprotection effects in B. malayi infection by maintaining a Th1–Th2 balance, evident from higher levels of IgG1 and IgG2a as well as IL-4 and IFN-γ. An improved understanding about the role of this filarial protein in host immunity, host–parasite interaction and worm clearance will aid in the development of good immunoprophylaxis for the disease. |
| Databáze: | OpenAIRE |
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