Microtubule dynamics in root hairs of Medicago truncatula
| Autor: | Antonius C.J. Timmers, Pascal Vallotton, Claudia Heym, Diedrik Menzel |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Lipopolysaccharides
Histology Green Fluorescent Proteins macromolecular substances Biology Root hair Microtubules Plant Roots Pathology and Forensic Medicine Protein filament Microtubule Medicago truncatula Tip growth Cytoskeleton Actin fungi food and beverages Cell Biology General Medicine biology.organism_classification Plant cell Actins Tubulin Modulators Cell biology Microscopy Fluorescence Rhizobium |
| Zdroj: | European Journal of Cell Biology. 86:69-83 |
| ISSN: | 0171-9335 |
| Popis: | The microtubular cytoskeleton plays an important role in the development of tip-growing plant cells, but knowledge about its dynamics is incomplete. In this study, root hairs of the legume Medicago truncatula have been chosen for a detailed analysis of microtubular cytoskeleton dynamics using GFP-MBD and EB1-YFP as markers and 4D imaging. The microtubular cytoskeleton appears mainly to be composed of bundles which form tracks along which new microtubules polymerise. Polymerisation rates of microtubules are highest in the tip of growing root hairs. Treatment of root hairs with Nod factor and latrunculin B result in a twofold decrease in polymerisation rate. Nonetheless, no direct, physical interaction between the actin filament cytoskeleton and microtubules could be observed. A new picture of how the plant cytoskeleton is organised in apically growing root hairs emerges from these observations, revealing similarities with the organisation in other, non-plant, tip-growing cells. |
| Databáze: | OpenAIRE |
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