Overlapping RdDM and non-RdDM mechanisms work together to maintain somatic repression of a paramutagenic epiallele of maize pericarp color1

Autor: Kameron Wittmeyer, Surinder Chopra, Po-Hao Wang, Tzuu-fen Lee, Blake C. Meyers
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Fruit and Seed Anatomy
lcsh:Medicine
Artificial Gene Amplification and Extension
Plant Science
medicine.disease_cause
Biochemistry
Polymerase Chain Reaction
Epigenesis
Genetic
Pericarp
Small interfering RNAs
lcsh:Science
Genetics
Regulation of gene expression
Mutation
Multidisciplinary
DNA methylation
Plant Anatomy
Eukaryota
Genomics
Plants
Chromatin
Nucleic acids
Enhancer Elements
Genetic
Experimental Organism Systems
Epigenetics
DNA modification
Chromatin modification
Research Article
Chromosome biology
Transposable element
Cell biology
Biology
Research and Analysis Methods
Genome Complexity
Genes
Plant
Zea mays
03 medical and health sciences
Model Organisms
Plant and Algal Models
medicine
Gene silencing
Grasses
Gene Silencing
Allele
Non-coding RNA
Molecular Biology Techniques
Gene
Molecular Biology
Alleles
Biology and life sciences
lcsh:R
Organisms
Computational Biology
DNA
Introns
Gene regulation
Maize
030104 developmental biology
Agronomy
RNA
lcsh:Q
Gene expression
Mutagens
Zdroj: PLoS ONE, Vol 12, Iss 11, p e0187157 (2017)
PLoS ONE
ISSN: 1932-6203
Popis: Allelic variation at the Zea mays (maize) pericarp color1 (p1) gene has been attributed to epigenetic gene regulation. A p1 distal enhancer, 5.2 kb upstream of the transcriptional start site, has demonstrated variation in DNA methylation in different p1 alleles/epialleles. In addition, DNA methylation of sequences within the 3' end of intron 2 also plays a role in tissue-specific expression of p1 alleles. We show here a direct evidence for small RNAs' involvement in regulating p1 that has not been demonstrated previously. The role of mediator of paramutation1 (mop1) was tested in the maintenance of somatic silencing at distinct p1 alleles: the non-paramutagenic P1-wr allele and paramutagenic P1-rr' epiallele. The mop1-1 mutation gradually relieves the silenced phenotype after multiple generations of exposure; P1-wr;mop1-1 plants display a loss of 24-nt small RNAs and DNA methylation in the 3' end of the intron 2, a region close to a Stowaway transposon. In addition, a MULE sequence within the proximal promoter of P1-wr shows depletion of 24nt siRNAs in mop1-1 plants. Release of silencing was not correlated with small RNAs at the distal enhancer region of the P1-wr allele. We found that the somatic silencing of the paramutagenic P1-rr' is correlated with significantly reduced H3K9me2 in the distal enhancer of P1-rr'; mop1-1 plants, while symmetric DNA methylation is not significantly different. This study highlights that the epigenetic regulation of p1 alleles is controlled both via RdDM as well as non-RdDM mechanisms.
Databáze: OpenAIRE
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