The Pineal Gland: A Supplemental Source of Hypothalamic-Releasing Hormones
| Autor: | E. S. Johnson, R. H. Rippel, M. T. Hedlund, John F. Wilber, W. F. White, G. F. Weber |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Ovulation
endocrine system medicine.medical_specialty Swine media_common.quotation_subject Radioimmunoassay Thyrotropin-releasing hormone Biology Tritium Pineal Gland Pineal gland Endocrinology Internal medicine medicine Animals Bioassay Thyrotropin-Releasing Hormone Melatonin media_common Estrous cycle Sheep Tissue Extracts Luteinizing Hormone Rats medicine.anatomical_structure Sephadex Pituitary Gland Chromatography Gel Cattle Female Follicle Stimulating Hormone hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Endocrinology. 94:1422-1426 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-94-5-1422 |
| Popis: | Crude extracts of commercially availa-ble ovine, bovine and porcine pineal glands were found to contain high levels of gonadotropin-releasing activity. The gonadotropin-releasing hor-mone (Gn-RH) radioimmunoassay values (porcine, 185 ng/gland; bovine, 500 ng/gland; and ovine, 490 ng/gland) were consistent with values obtained by in vitro Gn-RH bioassay. The pineal glands con-tained 4–10 times as much gonadotropin-releasing activity as that reported in hypothalami of the corresponding species. To verify this high ratio, fresh pineal glands and hypothalami were obtained from 4 ewes and similarly extracted. The pineal:hypothalamus activity ratio was found to be more than 20:1. The bovine and ovine pineal extracts were also tested for the induction of ovulation in the estrous rabbit. Ovulation occurred in all animals treated with the extract at a dose equivalent to 2μg Gn-RH (based on radioimmuno and bioassay results). One of the extracts was further characterized by gel filtration on Sephadex G-25 using 3H-p... |
| Databáze: | OpenAIRE |
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