Haloalkaliphilic Streptomyces spp. AJ8 isolated from solar salt works and its’ pharmacological potential

Autor: Mariathason Birdilla Selva Donio, Thavasimuthu Citarasu, Mariavincent Michaelbabu, John Selesteen Charles Adlin Jenifer, Samuel Gnana Prakash Vincent
Rok vydání: 2015
Předmět:
Zdroj: AMB Express
ISSN: 2191-0855
DOI: 10.1186/s13568-015-0143-2
Popis: Antagonistic Streptomyces spp. AJ8 was isolated and identified from the Kovalam solar salt works in India. The antimicrobial NRPS cluster gene was characterized by PCR, sequencing and predict the secondary structure analysis. The secondary metabolites will be extracted from different organic solvent extraction and studied the antibacterial, antifungal, antiviral and anticancer activities. In vitro antagonistic activity results revealed that, Streptomyces spp. AJ8 was highly antagonistic against Staphylococcus aureus, Aeromonas hydrophila WPD1 and Candida albicans. The genomic level identification revealed that, the strain was confirmed as Streptomyces spp. AJ8 and submitted the NCBI database (KC603899). The NRPS gene was generated a single gene fragment of 781 bp length (KR491940) and the database analysis revealed that, the closely related to Streptomyces spp. SAUK6068 and S. coeruleoprunus NBRC15400. The secondary metabolites extracted with ethyl acetate was effectively inhibited the bacterial and fungal growth at the ranged between 7 and 19.2 mm of zone of inhibition. The antiviral activity results revealed that, the metabolite was significantly (P
Databáze: OpenAIRE
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