RST1 and RIPR connect the cytosolic RNA exosome to the Ski complex in Arabidopsis
| Autor: | Michael Christie, Christina Piermaria, Nicolas Butel, Philippe Hammann, Patricia L. M. Lang, Johana Chicher, Carlos Gomez-Diaz, Detlef Weigel, Ezgi Süheyla Karaaslan, Hervé Vaucheret, David Pflieger, Lauriane Kuhn, Dominique Gagliardi, Heike Lange, Simon Y. A. Ndecky, Julie Zumsteg |
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| Přispěvatelé: | Institut de biologie moléculaire des plantes (IBMP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Génétique moléculaire, génomique, microbiologie (GMGM), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Développement de la protéomique comme outil d'investigation fonctionelle et d'annotation des génomes, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Génétique et de Biologie Moléculaire et Cellulaire, Université Louis Pasteur - Strasbourg I, Lange, Heike, Gagliardi, Dominique, Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), French National Research AgencyFrench National Research Agency (ANR), Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, ANR-17-EURE-0023,IMCBio,Integrative Molecular and Cellular Biology(2017) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Small RNA Small interfering RNA Exosome complex General Physics and Astronomy 02 engineering and technology 01 natural sciences nuclear-quality control RNA decay decay Arabidopsis Exoribonuclease Ski complex lcsh:Science degradation 0303 health sciences Multidisciplinary small interfering rnas core 021001 nanoscience & nanotechnology Cell biology siRNAs messenger-rnas components gene protein cuticular wax biosynthsis 0210 nano-technology animal structures Plant molecular biology Science Protein subunit Biology Exosome General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences [SDV.BV]Life Sciences [q-bio]/Vegetal Biology 030304 developmental biology RNA quality control RNA [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology General Chemistry biology.organism_classification 030104 developmental biology lcsh:Q human activities 010606 plant biology & botany |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019) Nature Communications Nature Communications, Nature Publishing Group, 2019, ⟨10.1038/s41467-019-11807-4⟩ Nature Communications (10), 1-12. (2019) Nature Communications, Nature Publishing Group, 2019, 10, pp.1-12. ⟨10.1038/s41467-019-11807-4⟩ Nature Communications, 2019, 10, pp.1-12. ⟨10.1038/s41467-019-11807-4⟩ |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-11807-4 |
| Popis: | The RNA exosome is a key 3’−5’ exoribonuclease with an evolutionarily conserved structure and function. Its cytosolic functions require the co-factors SKI7 and the Ski complex. Here we demonstrate by co-purification experiments that the ARM-repeat protein RESURRECTION1 (RST1) and RST1 INTERACTING PROTEIN (RIPR) connect the cytosolic Arabidopsis RNA exosome to the Ski complex. rst1 and ripr mutants accumulate RNA quality control siRNAs (rqc-siRNAs) produced by the post-transcriptional gene silencing (PTGS) machinery when mRNA degradation is compromised. The small RNA populations observed in rst1 and ripr mutants are also detected in mutants lacking the RRP45B/CER7 core exosome subunit. Thus, molecular and genetic evidence supports a physical and functional link between RST1, RIPR and the RNA exosome. Our data reveal the existence of additional cytosolic exosome co-factors besides the known Ski subunits. RST1 is not restricted to plants, as homologues with a similar domain architecture but unknown function exist in animals, including humans. Cytosolic RNA degradation by the RNA exosome requires the Ski complex. Here the authors show that the proteins RST1 and RIPR assist the RNA exosome and the Ski complex in RNA degradation, thereby preventing the production of secondary siRNAs from endogenous mRNAs. |
| Databáze: | OpenAIRE |
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