Validation of tumor-associated macrophage ferritin light chain as a prognostic biomarker in node-negative breast cancer tumors: A multicentric 2004 national PHRC study
| Autor: | Marie-Lise Jourdan, Antoine Carlioz, Pierre-Marie Martin, Agnès Chassevent, Frédérique Spyratos, Jean André, Marie-Pierre Joalland, Mario Campone, Delphine Loussouarn, Pascal Jézéquel, Catherine Guérin-Charbonnel, Catherine Grenot, Pascal Roy, Gabriel Ricolleau, Françoise Descotes, Loïc Campion |
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| Rok vydání: | 2011 |
| Předmět: |
Oncology
Adult Proteomics Cancer Research medicine.medical_specialty Pathology Epithelial-Mesenchymal Transition Antigens Differentiation Myelomonocytic Breast Neoplasms Tumor-associated macrophage Cohort Studies Breast cancer Cytosol Antigens CD Internal medicine medicine Biomarkers Tumor Humans Aged Cell Proliferation medicine.diagnostic_test business.industry CD68 Macrophages Middle Aged medicine.disease Prognosis Ferritin light chain Immunoassay Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Proteome Apoferritins Immunohistochemistry Female business |
| Zdroj: | International journal of cancer. 131(2) |
| ISSN: | 1097-0215 |
| Popis: | Novel prognostic biomarkers are imperatively needed to help direct treatment decisions by typing subgroups of node-negative breast cancer patients. Large screening of different biological compartments, such as the proteome, by means of high throughput techniques may greatly help scientists to find such markers. The present retrospective multicentric study included 268 node-negative breast cancer patients. We used a proteomic approach of SELDI-TOF-MS screening to identify differentially expressed cytosolic proteins with prognostic impact. The screening cohort was composed of 198 patients. Seventy supplementary patients were included for validation. Immunohistochemistry (IHC) and immunoassay (IA) were run to confirm the prognostic role of the marker identified by SELDI-TOF-MS screening. IHC was also used to explore links between selected marker and epithelial-mesenchymal transition (EMT)-like, proliferation and macrophage markers. Ferritin light chain (FTL) was identified as an independent prognostic marker (HR = 1.30-95% CI: 1.10-1.50, p = 0.001). Validation step by means of IHC and IA confirmed the prognostic value of FTL level. CD68 IHC showed that FTL was stored in tumor-associated macrophages (TAM), which exhibit an M2-like phenotype. We report here, first, the validation of FTL as a breast tumor prognostic biomarker in node-negative patients, and second, the fact that FTL is stored in TAM. |
| Databáze: | OpenAIRE |
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