Supplementary Methods, Figures 1-9 from CCL2 Mediates Cross-talk between Cancer Cells and Stromal Fibroblasts That Regulates Breast Cancer Stem Cells

Autor: Shizhen Emily Wang, Yukio Kuroki, Peggy Scherle, Hui Li, Xiubao Ren, Yuan-Zhong Wang, Yang Yu, Weiying Zhou, Shiuan Chen, Wei Ye, Xiwei Wu, Arthur Xuejun Li, Thehang Luu, Sofia Loera, Peiguo Chu, George Somlo, Jun Wu, Amy Chow, Akihiro Tsuyada
Rok vydání: 2023
Popis: PDF file - 111K, Methods: Includes Mammosphere formation assay, Cell transfection and reporter assays, RNAi studies, as well as Western blot analyses and xenograft data. Figures S1-S9 include the following: Marker expression in primary fibroblasts and BC cells (S1); Serial confocal images of representative mammospheres formed by PKH67-labeled BT474 cells in the presence or absence of CCL2 (S2); CCL2 expression in CAF3 cells upon CM treatments (S3); Sustained CCL2 induction in CAFs co-cultured with tumor cells (S4); Expression of CCR2 and CCR4 in BC cells (S5); HES1 and HEY1 reporter assays in BT474 cells (S6); In vitro secretase cleavage assay (S7); Representative IHC images of XP/CAF265922 xenograft tumors treated with PBS, IgG, or anti-CCL2 (S8); Representative IHC images of CCL2 and NOTCH1 in primary BC specimens (S9).
Databáze: OpenAIRE