Predicted ATP-binding cassette systems in the phytopathogenic mollicute Spiroplasma kunkelii
Autor: | Robert E. Davis, R. Jomantiene, Q. Liu, Shaoping Lin, Bruce A. Roe, Rosemarie W. Hammond, Hui Wang, Yan Zhao |
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Rok vydání: | 2004 |
Předmět: |
DNA Replication
DNA repair Spiroplasma Molecular Sequence Data Virulence ATP-binding cassette transporter Biology Zea mays Genome Frameshift mutation Adenosine Triphosphate Bacterial Proteins Phylogenetics Genetics Molecular Biology Gene Phylogeny Plant Diseases Whole genome sequencing Binding Sites General Medicine Protein Structure Tertiary Genes Bacterial ATP-Binding Cassette Transporters Genome Bacterial |
Zdroj: | Molecular Genetics and Genomics. 271:325-338 |
ISSN: | 1617-4623 1617-4615 |
DOI: | 10.1007/s00438-004-0983-y |
Popis: | Spiroplasma kunkelii is a cell wall-free, helical, and motile mycoplasma-like organism that causes corn stunt disease in maize. The bacterium has a compact genome with a gene set approaching the minimal complement necessary for cellular life and pathogenesis. A set of 21 ATP-binding cassette (ABC) domains was identified during the annotation of a draft S. kunkelii genome sequence. These 21 ABC domains are present in 18 predicted proteins, and are components of 16 functional systems, which account for 5% of the protein coding capacity of the S. kunkelii genome. Of the 16 systems, 11 are membrane-bound transporters, and two are cytosolic systems involved in DNA repair and the oxidative stress response; the genes for the remaining three hypothetical systems harbor nonsense and/or frameshift mutations, so their functional status is doubtful. Assembly of the 11 multicomponent transporters, and comparisons with other known systems permitted functional predictions for the S. kunkelii ABC transporter systems. These transporters convey a wide variety of substrates, and are critical for nutrient uptake, multidrug resistance, and perhaps virulence. Our findings provide a framework for functional characterization of the ABC systems in S. kunkelii. |
Databáze: | OpenAIRE |
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