Combining Two Technologies for Full Genome Sequencing of Human

Autor: N. M. Gruzdeva, Svetlana V. Tsygankova, David Zaridze, A M Mazur, V E Velikhov, D A Goranskaya, Artem V. Nedoluzhko, Sergey M. Rastorguev, Eugenia S. Boulygina, Egor Prokhortchouk, Anton B. Teslyuk, Konstantin G. Skryabin, Mikhail V. Kovalchuk, Vsevolod Matveev, Nikolay Chekanov
Rok vydání: 2009
Předmět:
Zdroj: Acta Naturae. 1:102-107
ISSN: 2075-8251
Popis: At present, the new technologies of DNA sequencing are rapidly developing allowing quick and efficient characterisation of organisms at the level of the genome structure. In this study, the whole genome sequencing of a human (Russian man) was performed using two technologies currently present on the market - Sequencing by Oligonucleotide Ligation and Detection (SOLiD™) (Applied Biosystems) and sequencing technologies of molecular clusters using fluorescently labeled precursors (Illumina). The total number of generated data resulted in 108.3 billion base pairs (60.2 billion from Illumina technology and 48.1 billion from SOLiD technology). Statistics performed on reads generated by GAII and SOLiD showed that they covered 75% and 96% of the genome respectively. Short polymorphic regions were detected with comparable accuracy however, the absolute amount of them revealed by SOLiD was several times less than by GAII. Optimal algorithm for using the latest methods of sequencing was established for the analysis of individual human genomes. The study is the first Russian effort towards whole human genome sequencing.
Databáze: OpenAIRE