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Zatoriamultiflora (Family) and Valerianawallichii (Family)both are wild herb and used in Unani system of medicine.Phytochemicals present in medicinal plant increase their usage so, that’s why widely used all over the world. The present study aimed to determine minimum and maximum reduction in colony growth of the crude extract and essential oil of aerial parts of Zatoria multiflora and roots of Valerianawallichiito control Shigellaboydii, Escherichia coli and Bacillus Subtilis. The crude extract and essential oil was tested in vitro with twelve concentrations: 0.5. 1.0, 1.5, 2.0, 2.5, and 3.0gm for crude extract and 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6ml for essential oil plus control using potato dextrose agar medium.Crude extract and essential oil of both plants showed significant antibacterial activity. The result showed with these evaluations, reduction in colonial growth was calculated by CFU method.The maximum reduction in colonial growth was up to 0.00mm at 0.6ml/LPDA dose of Zatoria multiflora essential oil against Shigellaboydii. While minimum reduction in colony growth noticed was 23mm at 0.5gm/PDA dose of Valerianawallichii against Shigellaboydii. Keywords: Zatoriamultiflora, Valerianawallichii, Crude extract, Essential oil, antibacterial activity. |
