Chronic corticosterone administration reduces dendritic complexity in mature, but not young granule cells in the rat dentate gyrus

Autor: Ang Li, Crystal A. Bostrom, Suk Yu Yau, Tatia M.C. Lee, Jian Bin Tong, Kwok-Fai So, Brian R. Christie
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
medicine.medical_specialty
Silver Staining
Neurogenesis
Anti-Inflammatory Agents
dendritic complexity
Hippocampal formation
Biology
Sholl analysis
Rats
Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Glucocorticoid receptor
Atrophy
Receptors
Glucocorticoid
Developmental Neuroscience
Western blot
Corticosterone
Internal medicine
medicine
Animals
Cells
Cultured
Neurons
Analysis of Variance
medicine.diagnostic_test
Golgi staining
Dentate gyrus
Granule (cell biology)
Gene Expression Regulation
Developmental
glucocorticoid receptors
Dendrites
medicine.disease
Rats
030104 developmental biology
Endocrinology
Neurology
chemistry
nervous system
Animals
Newborn
Dentate Gyrus
Neurology (clinical)
Neuroscience
030217 neurology & neurosurgery
Research Article
Zdroj: Restorative Neurology and Neuroscience
ISSN: 1878-3627
0922-6028
Popis: Background Our previous work has shown that exposure to the stress hormone corticosterone (40 mg/kg CORT) for two weeks induces dendritic atrophy of pyramidal neurons in the hippocampal CA3 region and behavioral deficits. However, it is unclear whether this treatment also affects the dentate gyrus (DG), a subregion of the hippocampus comprising a heterogeneous population of young and mature neurons. Objective We examined the effect of CORT treatment on the dendritic complexity of mature and young granule cells in the DG. Methods We utilized a Golgi staining method to investigate the dendritic morphology and spine density of young neurons in the inner granular cell layer (GCL) and mature neurons in the outer GCL in response to CORT application. The expressions of glucocorticoid receptors during neuronal maturation were examined using Western blot analysis in a primary hippocampal neuronal culture. Results Sholl analysis revealed that CORT treatment decreased the number of intersections and shortened the dendritic length in mature, but not young, granule cells. However, the spine density of mature and young neurons was not affected. Western blot analysis showed a progressive increase in the protein levels of glucocorticoid receptors (GRs) in the cultured primary hippocampal neurons during neuronal maturation. Conclusion These data suggest that mature neurons are likely more vulnerable to chronic exposure to CORT; this may be due to their higher expression of GRs when compared to younger DG neurons.
Databáze: OpenAIRE
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