Measuring the Induced Membrane Voltage with Di-8-ANEPPS
| Autor: | Damijan Miklavčič, Gorazd Pucihar, Tadej Kotnik |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Issue 33
potentiometric dyes electroporation Materials science induced transmembrane potential General Chemical Engineering electropermeabilization Lipid Bilayers Voltage-sensitive dye induced transmembrane voltage Pyridinium Compounds CHO Cells General Biochemistry Genetics and Molecular Biology Membrane Potentials Cell membrane Cricetulus Cricetinae Electric field medicine Animals Lipid bilayer Fluorescent Dyes Membrane potential induced membrane voltage General Immunology and Microbiology General Neuroscience Cell Membrane di-8-ANEPPS Fluorescence Cell biology Cellular Biology Membrane medicine.anatomical_structure Potentiometry Biophysics Voltage |
| Zdroj: | Journal of Visualized Experiments : JoVE |
| ISSN: | 1940-087X |
| DOI: | 10.3791/1659-v |
| Popis: | Placement of a cell into an external electric field causes a local charge redistribution inside and outside of the cell in the vicinity of the cell membrane, resulting in a voltage across the membrane. This voltage, termed the induced membrane voltage (also induced transmembrane voltage, or induced transmembrane potential difference) and denoted by DeltaPhi, exists only as long as the external field is present. If the resting voltage is present on the membrane, the induced voltage superimposes (adds) onto it. By using one of the potentiometric fluorescent dyes, such as di-8-ANEPPS, it is possible to observe the variations of DeltaPhi on the cell membrane and to measure its value noninvasively. di-8-ANEPPS becomes strongly fluorescent when bound to the lipid bilayer of the cell membrane, with the change of the fluorescence intensity proportional to the change of DeltaPhi. This video shows the protocol for measuring DeltaPhi using di-8-ANEPPS and also demonstrates the influence of cell shape on the amplitude and spatial distribution of DeltaPhi. |
| Databáze: | OpenAIRE |
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