N-Acylethanolamine Levels and Expression of Their Metabolizing Enzymes during Pregnancy
| Autor: | Bruno M. Fonseca, Stephen C. Bell, Timothy H. Marczylo, Anthony H. Taylor, Justin C. Konje, Georgina Correia-da-Silva, Patricia M.W. Lam, Natércia Teixeira |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Time Factors Gestational Age Biology Gene Expression Regulation Enzymologic Amidohydrolases chemistry.chemical_compound Endocrinology Western blot Pregnancy Internal medicine Cannabinoid Receptor Modulators Decidua Phospholipase D medicine Animals Decidual cells Embryo Implantation Rats Wistar medicine.diagnostic_test Decidualization Anandamide medicine.disease Endocannabinoid system Enzymes Rats medicine.anatomical_structure chemistry Cyclooxygenase 2 Ethanolamines Gestation Female |
| Zdroj: | Endocrinology. 151:3965-3974 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/en.2009-1424 |
| Popis: | Decidualization is essential for a successful pregnancy and is a tightly regulated process influenced by the local microenvironment. Lipid-based mediators, such as the endocannabinoid anandamide, and other compounds that have cannabimimetic actions may act on the decidua during early pregnancy. In this study, the levels of N-arachidonoylethanolamine (anandamide) and two other N-acylethanolamines, N-oleoylethanolamine and N-palmitoylethanolamine, were measured in rat plasma and maternal tissues between d 8 and 19 of pregnancy by ultraperformance liquid chromatography tandem mass spectrometry. The spatiotemporal expression of N-acylethanolamine metabolizing enzymes in implantation units were also determined by quantitative PCR, Western blot, and immunohistochemistry and shown to vary with gestation being mainly localized in decidual cells. The data also indicated that plasma and tissues levels of all three N-acylethanolamines fluctuate throughout pregnancy. Tissue levels of endocannabinoids did not correlate with plasma, suggesting that during pregnancy, maternal tissue levels of endocannabinoids are primarily regulated by in situ production and degradation to create endocannabinoid gradients conducive to successful pregnancy. |
| Databáze: | OpenAIRE |
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