Microencapsulation of new probiotic formulations for gastrointestinal delivery: in vitro study to assess viability and biological properties
| Autor: | Angela Michelotti, G. Bizzaro, S. Giardina, M. Boccarusso, Massimo Labra, P. Di Gennaro, B. La Ferla, I. Presti, Giuseppe D'Orazio |
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| Přispěvatelé: | D'Orazio, G, DI GENNARO, P, Boccarusso, M, Presti, I, Bizzaro, G, Giardina, S, Michelotti, A, Labra, M, LA FERLA, B |
| Rok vydání: | 2015 |
| Předmět: |
Oral
intestinal microbiota microencapsulated bacteria microencapsulation polymers probiotics Alginates Drug Compounding Microencapsulation polymer Administration Oral Capsules Settore BIO/19 - Microbiologia Generale Applied Microbiology and Biotechnology Models Biological law.invention Microbiology Probiotic chemistry.chemical_compound Lactobacillus rhamnosus Glucuronic Acid Models law Humans Food science Bifidobacterium Chitosan Microbial Viability biology Lacticaseibacillus rhamnosus Hexuronic Acids Probiotics General Medicine Settore CHIM/06 - Chimica Organica Biological biology.organism_classification Pleuran Bifidobacterium animalis Gastrointestinal Tract chemistry Administration Intestinal microbiota Microencapsulated bacteria Microencapsulation polymers Lactobacillus plantarum Bacteria Biotechnology |
| Zdroj: | Applied microbiology and biotechnology. 99(22) |
| ISSN: | 1432-0614 |
| Popis: | The paper describes the preparation of new probiotic formulations based on chitosan-coated alginate microcapsules containing three different probiotic strains, Lactobacillus plantarum PBS067, Lactobacillus rhamnosus PBS070, and Bifidobacterium animalis subsp. lactis PBS075 taken individually and as a mixture of them. The effects of microencapsulation on the viability of the strains in conditions simulating the gastrointestinal tract and under industrial processes conditions were studied. In addition, an evaluation of their probiotic properties was also investigated by in vitro tests on the human intestinal cell line HT-29 to explore the effect of microencapsulation on health beneficial effect of the considered strains. Non-encapsulated cells were completely destroyed when exposed to simulated gastric juice and other stress conditions, while encapsulated cells exhibited a significantly higher resistance to artificial intestinal juice and heat and osmotic treatment. Moreover, in this study, the effect of the various microencapsulated probiotic strain formulations was compared with analogous formulations also containing the β-glucan Pleuran. The microencapsulation effectively protected the selected bacteria, as single strain and as a mixture of the three strains in both the formulations with and without Pleuran, from simulating gastrointestinal tract and industrial process conditions in delivering the viable cells without any significant adverse effect on their functionalities. The comparative study of the immunomodulatory properties of each single strain and the mixture of the three strains revealed a synergistic effect of the probiotic mixture, but no appreciable difference between the two kinds of formulations could be detected, as the effect of Pleuran is covered by the higher potential of the probiotic strains. |
| Databáze: | OpenAIRE |
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