Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasion
| Autor: | John Marshall, Ann P. Wheeler, Shannon J L Pinnington |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Correlative Single Molecule Imaging/methods Materials science Confocal Resolution (electron density) Structured illumination microscopy Context (language use) Carcinoma Squamous Cell/pathology Lighting/instrumentation Mouth Neoplasms/pathology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Imaging Three-Dimensional/methods Podosomes/pathology Invadopodia Microscopy Tumor Cells Cultured Humans Neoplasm Invasiveness Nanoscopic scale 030217 neurology & neurosurgery Microscopy Fluorescence/methods Biomedical engineering |
| Zdroj: | Pinnington, S J L, Marshall, J F & Wheeler, A P 2018, Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasion . in Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasio . vol. 1764, Methods in molecular biology (Clifton, N.J.), pp. 253-265 . https://doi.org/10.1007/978-1-4939-7759-8_15 Protein Complex Assembly ISBN: 9781493977581 |
| DOI: | 10.1007/978-1-4939-7759-8_15 |
| Popis: | Super-resolution microscopy methods enable resolution of biological molecules in their cellular or tissue context at the nanoscale. Different methods have their strengths and weaknesses. Here we present a method that enables correlative confocal, structured illumination microscopy (SIM) and single-molecule localization microscopy (SMLM) imaging of structures involved in formation of invadopodia on the same sample. This enables up to four colors to be visualized in three dimensions at a resolution of between 120 and 10 nm for SIM and SMLM, respectively. |
| Databáze: | OpenAIRE |
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