Identification and widespread environmental distribution of a gene cassette implicated in anaerobic dichloromethane degradation

Autor: Robert W. Murdoch, Gao Chen, Fadime Kara Murdoch, E. Erin Mack, Manuel I. Villalobos Solis, Robert L. Hettich, Frank E. Löffler
Rok vydání: 2022
Předmět:
Zdroj: Global Change Biology. 28:2396-2412
ISSN: 1365-2486
1354-1013
DOI: 10.1111/gcb.16068
Popis: Anthropogenic activities and natural processes release dichloromethane (DCM, methylene chloride), a toxic chemical with substantial ozone-depleting capacity. Specialized anaerobic bacteria metabolize DCM; however, the genetic basis for this process has remained elusive. Comparative genomics of the three known anaerobic DCM-degrading bacterial species revealed a homologous gene cluster, designated the methylene chloride catabolism (mec) gene cassette, comprising 8-10 genes encoding proteins with 79.6%-99.7% amino acid identities. Functional annotation identified genes encoding a corrinoid-dependent methyltransferase system, and shotgun proteomics applied to two DCM-catabolizing cultures revealed high expression of proteins encoded on the mec gene cluster during anaerobic growth with DCM. In a DCM-contaminated groundwater plume, the abundance of mec genes strongly correlated with DCM concentrations (R
Databáze: OpenAIRE
Externí odkaz: