Isolation and characterization of SSR and EST-SSR loci in Chamaecyparis formosensis (Cupressaceae)
| Autor: | Shau-Chian Liu, Yi-Shiang Huang, Fang-Hua Chu, Chi-Tsong Chen, Li-Ting Ma, Chi-Hsiang Chao, Yu-Hsin Tseng, Hsiang-Chih Hsieh, Shuo-Yu Hsu, Chiun-Jr Huang, Chieh-Ting Wang, Ya Ting You |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Chamaecyparis formosensis Cupressaceae Genomics Plant Science Biology 010603 evolutionary biology 01 natural sciences Loss of heterozygosity 03 medical and health sciences Primer Notes Cypress Ecology Evolution Behavior and Systematics Genetics Expressed sequence tag food and beverages Primer Note biology.organism_classification expressed sequence tag–simple sequence repeat (EST‐SSR) marker simple sequence repeat (SSR) marker 030104 developmental biology Genetic marker Microsatellite |
| Zdroj: | Applications in Plant Sciences |
| ISSN: | 2168-0450 |
| DOI: | 10.1002/aps3.1175 |
| Popis: | Premise of the study Simple sequence repeat (SSR) and expressed sequence tag (EST)-SSR markers were developed as tools for marker-assisted selection of Chamaecyparis formosensis and for the molecular differentiation of cypress species. Methods and results Based on the SSR-enriched genomic libraries and transcriptome data of C. formosensis, 300 primer pairs were selected for initial confirmation, of which 19 polymorphic SSR and eight polymorphic EST-SSR loci were chosen after testing in 92 individuals. The number of alleles observed for these 27 loci ranged from one to 17. The levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.000 to 0.903, respectively. Most markers also amplified in C. obtusa var. formosana. Conclusions The developed SSR and EST-SSR sequences are the first reported markers specific to C. formosensis. These markers will be useful for individual identification of C. formosensis and to distinguish cypress species such as C. obtusa var. formosana. |
| Databáze: | OpenAIRE |
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