Diffusion-mediated HEI10 coarsening can explain meiotic crossover positioning in Arabidopsis
Autor: | Ian R. Henderson, Chris Morgan, Matthew Hartley, Martin Howard, John A. Fozard, Kirsten Bomblies |
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Přispěvatelé: | Morgan, Chris [0000-0002-7475-2155], Fozard, John A. [0000-0001-9181-8083], Howard, Martin [0000-0001-7670-0781], Apollo - University of Cambridge Repository, Fozard, John A [0000-0001-9181-8083] |
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Plant genetics
Chromosomal Proteins Non-Histone Science Crossover Arabidopsis Gene Dosage General Physics and Astronomy Interference (genetic) Chromosomes Plant General Biochemistry Genetics and Molecular Biology Chromosomal crossover Cytogenetics 03 medical and health sciences 0302 clinical medicine Meiosis 631/114/2397 Computational models Arabidopsis thaliana Computer Simulation Crossing Over Genetic Diffusion (business) 030304 developmental biology Physics 0303 health sciences Multidisciplinary biology Synaptonemal Complex Arabidopsis Proteins article 631/208/1405 General Chemistry biology.organism_classification Synaptonemal complex 631/449/2491 14/63 Biophysics Pachytene Stage 030217 neurology & neurosurgery |
Zdroj: | Nature Communications, 12 Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021) Nature Communications |
ISSN: | 2041-1723 |
DOI: | 10.3929/ethz-b-000500965 |
Popis: | In most organisms, the number and distribution of crossovers that occur during meiosis are tightly controlled. All chromosomes must receive at least one ‘obligatory crossover’ and crossovers are prevented from occurring near one another by ‘crossover interference’. However, the mechanistic basis of this phenomenon of crossover interference has remained mostly mysterious. Using quantitative super-resolution cytogenetics and mathematical modelling, we investigate crossover positioning in the Arabidopsis thaliana wild-type, an over-expressor of the conserved E3 ligase HEI10, and a hei10 heterozygous line. We show that crossover positions can be explained by a predictive, diffusion-mediated coarsening model, in which large, approximately evenly-spaced HEI10 foci grow at the expense of smaller, closely-spaced clusters. We propose this coarsening process explains many aspects of Arabidopsis crossover positioning, including crossover interference. Consistent with this model, we also demonstrate that crossover positioning can be predictably modified in vivo simply by altering HEI10 dosage, with higher and lower dosage leading to weaker and stronger crossover interference, respectively. As HEI10 is a conserved member of the RING finger protein family that functions in the interference-sensitive pathway for crossover formation, we anticipate that similar mechanisms may regulate crossover positioning in diverse eukaryotes. Nature Communications, 12 ISSN:2041-1723 |
Databáze: | OpenAIRE |
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