Magnesium modulates parathyroid hormone secretion and upregulates parathyroid receptor expression at moderately low calcium concentration
| Autor: | Kristina Gundlach, Antonio Canalejo, Mirjam E. Peter, Juan F. Navarro-González, Pablo Perez-Martinez, Yolanda Almaden, Alan Peralta-Ramírez, María E. Rodríguez-Ortiz, Sonja Steppan, Jutta Passlick-Deetjen, Mariano Rodriguez, Julio M. Martinez-Moreno, Juan R. Muñoz-Castañeda, Carmen Herencia |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
calcium-sensing receptor Receptor expression Parathyroid hormone Gene Expression Magnesium Compounds magnesium Real-Time Polymerase Chain Reaction Calcitriol receptor Phosphates Parathyroid Glands Basic Science Internal medicine Medicine vitamin D receptor Animals RNA Messenger Receptor Fibroblast Growth Factor Type 1 Rats Wistar Klotho Cells Cultured Receptor Parathyroid Hormone Type 1 Calcium metabolism Transplantation business.industry Parathyroid chief cell Original Articles Immunohistochemistry Rats Up-Regulation Disease Models Animal Endocrinology Nephrology Parathyroid Hormone Parathyroid hormone secretion Receptors Calcitriol Calcium Calcium-sensing receptor business Receptors Calcium-Sensing hormones hormone substitutes and hormone antagonists PTH |
| Zdroj: | Nephrology Dialysis Transplantation |
| ISSN: | 1460-2385 0931-0509 |
| Popis: | Background The interest on magnesium (Mg) has grown since clinical studies have shown the efficacy of Mg-containing phosphate binders. However, some concern has arisen for the potential effect of increased serum Mg on parathyroid hormone (PTH) secretion. Our objective was to evaluate the direct effect of Mg in the regulation of the parathyroid function; specifically, PTH secretion and the expression of parathyroid cell receptors: CaR, the vitamin D receptor (VDR) and FGFR1/Klotho. Methods The work was performed in vitro by incubating intact rat parathyroid glands in different calcium (Ca) and Mg concentrations. Results Increasing Mg concentrations from 0.5 to 2 mM produced a left shift of PTH-Ca curves. With Mg 5 mM, the secretory response was practically abolished. Mg was able to reduce PTH only if parathyroid glands were exposed to moderately low Ca concentrations; with normal-high Ca concentrations, the effect of Mg on PTH inhibition was minor or absent. After 6-h incubation at a Ca concentration of 1.0 mM, the expression of parathyroid CaR, VDR, FGFR1 and Klotho (at mRNA and protein levels) was increased with a Mg concentration of 2.0 when compared with 0.5 mM. Conclusions Mg reduces PTH secretion mainly when a moderate low calcium concentration is present; Mg also modulates parathyroid glands function through upregulation of the key cellular receptors CaR, VDR and FGF23/Klotho system. |
| Databáze: | OpenAIRE |
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