A direct protein kinase B-targeted anti-inflammatory activity of cordycepin from artificially cultured fruit body of Cordyceps militaris
| Autor: | Seung-Eun Lee, Ju Young Yoon, Je Hun Choi, Jae Youl Cho, Dae Young Lee, Kwang Soo Baek, Kang Ro Lee, Geum Soog Kim, Hyun Bong Park, Gi-Ho Sung, Seung Yu Kim, Hyung Jun Noh, Ji Hye Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
cordycepin Cordycepin Cordyceps militaris (Clavicipitaceae) Linoleic acid Pharmaceutical Science Biology biology.organism_classification Palmitic acid Butyric acid chemistry.chemical_compound Anti-inflammatory activity Enzyme chemistry Biochemistry Drug Discovery Cordyceps militaris phytochemical study protein kinase B Original Article Kinase activity Protein kinase B |
| Zdroj: | Pharmacognosy Magazine |
| ISSN: | 0976-4062 0973-1296 |
| Popis: | Background: Cordyceps militaris is one of well-known medicinal mushrooms with anti-inflammatory, anti-cancer, anti-diabetic, and anti-obesity activities. Objective: The objective of the following study is to isolate chemical components from the ethanol extract (Cm-EE) from Cordyceps militaris and to evaluate their anti-inflammatory activities. Materials and Methods: Column chromatographic separation was performed and anti-inflammatory roles of these compounds were also examined by using NO production and protein kinase B (AKT) activity assays. Results: From Cm-EE, 13 constituents, including trehalose (1), cordycepin (2), 6-hydroxyethyladenosine (3), nicotinic amide (4), butyric acid (5), β-dimorphecolic acid (6), α-dimorphecolic acid (7), palmitic acid (8), linoleic acid (9), cordycepeptide A (10), 4-(2-hydroxy-3-((9E,12E)-octadeca-9,12-dienoyloxy)propoxy)-2-(trimethylammonio)butanoate (11), 4-(2-hydroxy-3-(palmitoyloxy)propoxy)-2-(trimethylammonio)butanoate (12), and linoleic acid methyl ester (13) were isolated. Of these components, compound 2 displayed a significant inhibitory effect on NO production in lipopolysaccharide (LPS)-activated RAW264.7 cells. Furthermore, this compound strongly and directly suppressed the kinase activity of AKT, an essential signalling enzyme in LPS-induced NO production, by interacting with its ATP binding site. Conclusion: C. militaris could have anti-inflammatory activity mediated by cordycepin-induced suppression of AKT. |
| Databáze: | OpenAIRE |
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