Modeling Barrier Properties of Intestinal Mucus Reinforced with IgG and Secretory IgA against Motile Bacteria
| Autor: | Alex Chen, Samuel K. Lai, Timothy Wessler, Holly A. Schroeder, Jay M. Newby, Jennifer L. Schiller, Feifei Xu, M. Gregory Forest |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Agglutination Motile bacteria 030106 microbiology Microbiology 03 medical and health sciences Animals Humans Secretory IgA Intestinal Mucosa Binding Sites Intestinal mucus Bacteria biology Chemistry Mucin Mucins Models Theoretical Mucus 030104 developmental biology Infectious Diseases Immunoglobulin G Immunoglobulin A Secretory biology.protein Antibody Protein Binding |
| Zdroj: | ACS Infectious Diseases. 5:1570-1580 |
| ISSN: | 2373-8227 |
| DOI: | 10.1021/acsinfecdis.9b00109 |
| Popis: | The gastrointestinal (GI) tract is lined with a layer of viscoelastic mucus gel, characterized by a dense network of entangled and cross-linked mucins together with an abundance of antibodies (Ab). Secretory IgA (sIgA), the predominant Ab isotype in the GI tract, is a dimeric molecule with 4 antigen-binding domains capable of inducing efficient clumping of bacteria, or agglutination. IgG, another common Ab at mucosal surfaces, can cross-link individual viruses to the mucin mesh through multiple weak bonds between IgG-Fc and mucins, a process termed muco-trapping. Relative contributions by agglutination versus muco-trapping in blocking permeation of motile bacteria through mucus remain poorly understood. Here, we developed a mathematical model that takes into account physiologically relevant spatial dimensions and time scales, binding and unbinding rates between Ab and bacteria as well as between Ab and mucins, the diffusivities of Ab, and run-tumble motion of active bacteria. Our model predicts both sIgA and IgG can accumulate on the surface of individual bacteria at sufficient quantities and rates to enable trapping individual bacteria in mucins before they penetrate the mucus layer. Furthermore, our model predicts that agglutination only modestly improves the ability for antibodies to block bacteria permeation through mucus. These results suggest that while sIgA is the most potent Ab isotype overall at stopping bacterial penetration, IgG may represent a practical alternative for mucosal prophylaxis and therapy. Our work improves the mechanistic understanding of Ab-enhanced barrier properties of mucus and highlights the ability for muco-trapping Ab to protect against motile pathogens at mucosal surfaces. |
| Databáze: | OpenAIRE |
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