Effects of hydrogen peroxide and hydroxyl radicals on the cytosolic side of a non-selective cation channel in the cultured human bronchial epithelial cell line 16HBE14o
| Autor: | Anne-Catherine Dazy, Claudette Jeulin, Francelyne Marano |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Physiology
Radical Clinical Biochemistry Respiratory Mucosa medicine.disease_cause Ion Channels Membrane Potentials chemistry.chemical_compound Cytosol Physiology (medical) Cations Extracellular medicine Humans Cells Cultured chemistry.chemical_classification Reactive oxygen species Chemistry Hydroxyl Radical Epithelial Cells Hydrogen Peroxide Oxidants Electrophysiology Biochemistry Apoptosis Biophysics Hydroxyl radical Calcium Intracellular Oxidative stress |
| Zdroj: | Pflugers Archiv : European journal of physiology. 443(4) |
| ISSN: | 0031-6768 |
| Popis: | Respiratory tissues can be damaged by the exposure of airway epithelial cells to reactive oxygen species (ROS) that generate an oxidative stress. We studied the effects of the hydroxyl radical *OH, for which there is no natural intra- or extracellular scavenger, on a Ca2+-activated, non-selective cation channel (NSC(Ca)) which might participate in the transepithelial Na+ fluxes involved in the maintenance of the cytosolic [Na+] ([Na+]i). We identified and characterized NSC(Ca) in inside-out excised membrane patches from cells of the human bronchial cell line 16HBE14o- and exposed the cytoplasmic side of NSC(Ca) to H2O2 or *OH created in front of the patch pipette. In these cells, the NSC channel was Ca2+ (above 0.1 microM) and voltage dependent. The channel's low open probability ( P(o)) at hyperpolarized voltages increased rapidly to a very high value upon short exposure (seconds) to *OH or to H2O2 in a bath solution containing millimolar [Ca2+]. This sensibility to ROS was reversible on wash-out of the oxidants. Long exposure (several minutes) to *OH in a bath solution containing 10(-7) M Ca2+ activated the channel irreversibly, a finding that has pathophysiological implications. The functioning of the NSC(Ca) protein channel may be modified by the intracellular redox status and the reversibility of the sensitivity to ROS depends on [Ca2+]i. The role of NSC(Ca) channels in airway epithelia is not yet understood, but might conform to what might be expected of an NSC channel: increase in [Na]i following intracellular exposure to *OH or H2O2. Reversible or irreversible excessive opening of cation channels may induce gradual cellular responses, including changes in Na+/K+-ATPase activity, cell volume regulation, membrane polarization, activation of transcription factors required for the expression of genes of cytokines or chemokines, apoptosis and necrosis, thus triggering inflammation. |
| Databáze: | OpenAIRE |
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