Functional Analysis of the Promoter Regions of Two Apoptosis-Related Genes (Bcl-2 and Cycs) and Their Regulation by Zn in Yellow Catfish
| Autor: | Chang-Chun Song, Fang Chen, Yang He, Chong-Chao Zhong, Zhi Luo, Tao Zhao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
QH301-705.5 Article Catalysis Inorganic Chemistry 03 medical and health sciences 0302 clinical medicine Transcriptional regulation Animals Zn transcriptional regulation RNA Messenger Biology (General) Physical and Theoretical Chemistry Promoter Regions Genetic STAT3 QD1-999 Molecular Biology Transcription factor Catfishes Spectroscopy Sequence Deletion Binding Sites Base Sequence biology promoter analysis Chemistry Cytochrome c Organic Chemistry apoptosis Cytochromes c Promoter General Medicine Molecular biology Computer Science Applications DNA binding site Zinc Hepatocyte nuclear factors 030104 developmental biology Gene Expression Regulation Proto-Oncogene Proteins c-bcl-2 030220 oncology & carcinogenesis Hepatocytes STAT protein biology.protein vertebrates |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 12 International Journal of Molecular Sciences, Vol 22, Iss 6291, p 6291 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22126291 |
| Popis: | B-cell lymphoma 2 (Bcl-2) and cytochrome c (Cycs) are two important proteins relevant to cellular apoptosis. In this study, we characterized the functions of the promoter regions of two apoptosis-related genes, Bcl-2 and Cycs, in yellow catfish Pelteobagrus fulvidraco. We obtained a 1989 bp Bcl-2 promoter and an 1830 bp Cycs promoter and predicted several key transcription factor binding sites (TFBSs) on the promoters, such as Kruppel-like factor 4 (KLF4), signal transducer and activator of transcription factor 3 (STAT3), forkhead box O (FOXO), metal-responsive element (MRE) and hepatocyte nuclear factor 1α (HNF-1α). Zinc (Zn) increased the activities of the Bcl-2 promoter but decreased the activities of the Cycs promoter. Metal-responsive transcription factor 1 (MTF-1) and HNF-1α directly bound with Bcl-2 and Cycs promoters, and they positively regulated the activity of the Bcl-2 promoter but negatively regulated the activity of the Cycs promoter. Zn promoted the binding ability of HNF-1α to the Bcl-2 promoter but decreased its binding ability to the Cycs promoter. However, Zn had no significant effect on the binding capability of MTF-1 to the regions of Bcl-2 and Cycs promoters. Zn upregulated the mRNA and total protein expression of Bcl-2 but downregulated the mRNA and total protein expression of Cycs. At the same time, Annexin V–FITC/PI staining showed that Zn significantly reduced the apoptosis of primary hepatocytes. For the first time, our study provides evidence for the MRE and HNF-1α response elements on the Bcl-2 and Cycs promoters, offering new insight into the mechanism by which Zn affects apoptosis in vertebrates. |
| Databáze: | OpenAIRE |
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