A survey ofCampylobacterspecies shed in faeces of beef cattle using polymerase chain reaction
| Autor: | G D Inglis, H.W. Busz, Lisa D. Kalischuk |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
DNA
Bacterial Immunology Cattle Diseases Campylobacteriosis Beef cattle medicine.disease_cause Polymerase Chain Reaction Applied Microbiology and Biotechnology Microbiology law.invention Feces law Campylobacter Infections Prevalence Genetics medicine Animals Molecular Biology Polymerase chain reaction DNA Primers biology Campylobacter General Medicine biology.organism_classification medicine.disease Campylobacter hyointestinalis Campylobacter coli Cattle Campylobacter fetus Primer (molecular biology) |
| Zdroj: | Canadian Journal of Microbiology. 49:655-661 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/w03-087 |
| Popis: | A polymerase chain reaction (PCR)-based survey of campylobacters associated with faeces collected from 382 beef cattle was undertaken. To ensure the removal of PCR inhibitors present in faeces and determine if adequate extraction was achieved, faeces were seeded with internal control DNA (i.e., DNA designed to amplify with the Campylobacter genus primer set, but provide a smaller amplicon) before the extraction procedure. In only two samples (0.5%) were the internal control or Campylobacter genus amplicons not detected. In the remaining 380 faecal samples, Campylobacter DNA was detected in 83% of the faecal samples (80% of the faecal samples were positive for Campylobacter genus DNA, and 3% of the samples were negative for Campylobacter genus DNA but positive for DNA of individual species). The most frequently detected species was Campylobacter lanienae (49%), a species only recently connected to livestock hosts. Campylobacter jejuni DNA was detected in 38% of the faecal samples, and Campylobacter hyointestinalis and Campylobacter coli DNA were detected in 8% and 0.5% of the samples, respectively. Campylobacter fetus DNA was not detected. Twenty-four percent of the faecal samples contained DNA of at least two species of Campylobacter. Of these samples, the majority (81%) contained DNA of C. jejuni and C. lanienae. The results of this study indicate that beef cattle commonly release a variety of Campylobacter species into the environment and may contribute to the high prevalence of campylobacteriosis in humans inhabiting areas of intensive cattle production, such as southern Alberta. Furthermore, this study demonstrates the utility of using PCR as a rapid and accurate method for simultaneously detecting the DNA of a diverse number of Campylobacter species associated with bovine faeces.Key words: campylobacters, detection, technique, Bos taurus. |
| Databáze: | OpenAIRE |
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