Identification of the phytosphingosine metabolic pathway leading to odd-numbered fatty acids
| Autor: | Takashi Obara, Akio Kihara, Maki Yamagata, Takuya Kitamura, Natsuki Kondo, Tatsuro Naganuma, Naoya Seki, Yusuke Ohno |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Molecular Sequence Data General Physics and Astronomy Acetate-CoA Ligase Lyases CHO Cells Palmitic Acids Saccharomyces cerevisiae Glycerophospholipids Biology General Biochemistry Genetics and Molecular Biology Phosphates Mice Cricetulus Sphingosine Cricetinae Animals Humans Amino Acid Sequence chemistry.chemical_classification Sphingolipids Multidisciplinary Fatty Acids Fatty acid General Chemistry Metabolism Sphingolipid Yeast Metabolic pathway HEK293 Cells chemistry Biochemistry Free fatty acid receptor Metabolic Networks and Pathways Polyunsaturated fatty acid |
| Zdroj: | Nature communications. 5 |
| ISSN: | 2041-1723 |
| Popis: | The long-chain base phytosphingosine is a component of sphingolipids and exists in yeast, plants and some mammalian tissues. Phytosphingosine is unique in that it possesses an additional hydroxyl group compared with other long-chain bases. However, its metabolism is unknown. Here we show that phytosphingosine is metabolized to odd-numbered fatty acids and is incorporated into glycerophospholipids both in yeast and mammalian cells. Disruption of the yeast gene encoding long-chain base 1-phosphate lyase, which catalyzes the committed step in the metabolism of phytosphingosine to glycerophospholipids, causes an similar to 40% reduction in the level of phosphatidylcholines that contain a C15 fatty acid. We also find that 2-hydroxypalmitic acid is an intermediate of the phytosphingosine metabolic pathway. Furthermore, we show that the yeast MPO1 gene, whose product belongs to a large, conserved protein family of unknown function, is involved in phytosphingosine metabolism. Our findings provide insights into fatty acid diversity and identify a pathway by which hydroxyl group-containing lipids are metabolized. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|