Donor cell microchimerism in kidney transplantation: Implications for graft function
| Autor: | Mahmoud Zidan, Gamal Saadi, May Hassaballa, Walaa Abdel Fattah, Ayda K. Kelany, Mervat El-Ansary, Mariam Onsy F. Hanna |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Graft Rejection Male medicine.medical_specialty Immunology Renal function Gastroenterology Chimerism Polymerase Chain Reaction Young Adult Immune system Internal medicine Genetics medicine Humans Renal Insufficiency Chronic Molecular Biology Genetics (clinical) Kidney transplantation Whole blood Transplantation Chimera business.industry Graft Survival Microchimerism General Medicine medicine.disease Kidney Transplantation Tissue Donors Transplantation surgical procedures operative Real-time polymerase chain reaction Female business Biomarkers Kidney disease Follow-Up Studies |
| Zdroj: | International journal of immunogeneticsREFERENCES. 47(6) |
| ISSN: | 1744-313X |
| Popis: | Given the uncertainty regarding the relationship between donor cells at microchimeric levels and its influence on graft function and clinical outcome, we explored the extent and importance of donor microchimerism in kidney transplantation. Twenty patients with chronic kidney disease who had received allografts from living donors were studied. We examined peripheral whole blood samples from the recipients one month after the transplant, applying mitochondrial DNA variant-specific polymerase chain reaction (PCR) to identify and quantify donor cells in relation to allograft function and survival during three years of follow-up. Higher quantities of donor-derived cell microchimerism in the peripheral blood correlated with better graft function in the early postoperative period at 1 month (R2 = .536, p = .001) and predicted improved graft function 1 year following the transplant (R2 = .430, p = .008). Furthermore, early post-transplant quantities of donor cell microchimerism were an important predictor of improved kidney function 3 years after transplantation (R2 = .397, p = .021). However, donor cell microchimerism failed to predict patient and graft survival after 3 years (odds ratio = 0.536, p = .860). Our findings suggest that donor cell microchimerism plays an immunoregulatory role in kidney transplantation and contributes to donor-specific immune hypo-responsiveness and graft acceptance. |
| Databáze: | OpenAIRE |
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