| Popis: |
Mammalian erythrocytes contain a pyruvic phosphoferase (PPFase) which is activated by K+and Mg++and inhibited by Na+and Ca++. The K+can be replaced by Rb+or NH4+, and the Mg++can be partially replaced by Mn++or Co++as activators of the enzyme. The PPFase apparently requires free thiol groups for its activity, as it is completely inhibited by 10−4 M p-chloromercuribenzoate and this inhibition is partially reversed by glutathione. The mean PPFase of the erythrocytes of six mammalian species was determined and found to be in the following order of decreasing activity: man, rat, dog, rabbit, cat, ox. The erythrocytes of chicks and of newborn rats showed a very high PPFase activity. |
