CtBP/BARS induces fission of Golgi membranes by acylating lysophosphatidic acid
| Autor: | Mario Salmona, Elena V. Polishchuk, Maria Giuseppina Silletta, Daniela Corda, Gabriele Turacchio, Claudia Cericola, Stefania Spanò, Antonino Colanzi, Koert N.J. Burger, Roberto Weigert, Silvia Senatore, Francesco Facchiano, Raffaella Mancini, Alexander A. Mironov, Alberto Luini |
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| Předmět: |
Fission
Acylation Golgi Apparatus Biology In Vitro Techniques chemistry.chemical_compound symbols.namesake Membrane Lipids Membrane fission Lysophosphatidic acid Animals Multidisciplinary Brain Phosphatidic acid Intracellular Membranes Golgi apparatus Membrane transport Recombinant Proteins Transport protein Rats Membrane Biochemistry chemistry Biophysics symbols lipids (amino acids peptides and proteins) Acyl Coenzyme A Lysophospholipids Carrier Proteins Transcription Factors |
| Zdroj: | ResearcherID |
| Popis: | Membrane fission is essential in intracellular transport. Acyl-coenzyme As (acyl-CoAs) are important in lipid remodelling and are required for fission of COPI-coated vesicles1. Here we show that CtBP/BARS, a protein that functions in the dynamics of Golgi tubules2, is an essential component of the fission machinery operating at Golgi tubular networks, including Golgi compartments involved in protein transport and sorting. CtBP/BARS-induced fission was preceded by the formation of constricted sites in Golgi tubules, whose extreme curvature is likely to involve local changes in the membrane lipid composition. We find that CtBP/BARS uses acyl-CoA to selectively catalyse the acylation of lysophosphatidic acid to phosphatidic acid both in pure lipidic systems and in Golgi membranes, and that this reaction is essential for fission. Our results indicate a key role for lipid metabolic pathways in membrane fission. |
| Databáze: | OpenAIRE |
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