HLA-DR expression is induced on keratinocytes in delayed hypersensitivity but not in allergen induced late-phase reactions
| Autor: | M. C. J. M. De Jong, Henk M. Jansen, K. Heeres, M. S. Weller, J. G. R. De Monchy, F. R. Weller |
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| Přispěvatelé: | Other departments |
| Rok vydání: | 1995 |
| Předmět: |
CD4-Positive T-Lymphocytes
Keratinocytes Allergy medicine.medical_treatment Immunology medicine.disease_cause Immunophenotyping Immunoenzyme Techniques Allergen Antigen HLA-DR medicine Animals Humans Immunology and Allergy Hypersensitivity Delayed Antigens Dermatophagoides Glycoproteins Skin Skin Tests House dust mite Mites integumentary system biology Helix Snails Hemocyanin HLA-DR Antigens Allergens biology.organism_classification medicine.disease Eosinophils medicine.anatomical_structure Delayed hypersensitivity Dermatitis Allergic Contact Hemocyanins Keratinocyte |
| Zdroj: | Clinical and experimental allergy, 25(3), 252-259. Wiley-Blackwell |
| ISSN: | 1365-2222 0954-7894 |
| Popis: | In view of increasing evidence suggesting an active immunoregulatory role of the skin keratinocytes and the observation that the differentiation of allergen specific T lymphocytes is critical in the development of allergy, we evaluated epidermal expression of HLA-DR antigen in skin reactions induced with an atopen (house dust mite) and with an non-atopic antigen (Hemocyanin). Two groups of patients with house dust mite (Dermatophagoides pteronyssinus [Der p]) allergy were compared, one group was skin tested with Der p, the other group was immunized and subsequently skin tested with Helix pomatia Hemocyanin (HPH). Biopsy specimens taken at 48 h after the HPH (n = 11) and Der p (n = 11) tests were analysed immunohistologically. Reactions in both groups were comparable in size. Immunohistological analysis showed domination by CD4+ lymphocytes. Expression of HLA-DR antigen by epidermal keratinocytes was observed in six out of 11 of the HPH induced reactions, but in none of the Der p induced reactions. Eosinophils were spotted only throughout the Der p induced reactions, showing a good correlation with the number of CD4 positive lymphocytes. The lack of HLA-DR expression by keratinocytes during the allergen-induced reaction, compared with the Hemocyanin induced reaction can be the result of a difference in cytokine profile of the lymphocytes dominating the dermal infiltrate. On the other hand evidence exists that defective HLA-DR expression by keratinocytes enhances antigen induced lymphocyte activation, and may thus contribute to the development of allergen-specific T-lymphocytes. |
| Databáze: | OpenAIRE |
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