Evidence for Four-Component Close-Range Sex Pheromone in the Parasitic Wasp Glyptapanteles flavicoxis

Autor: Regine Gries, Paul W. Schaefer, Gerhard Gries, Adela Danci
Rok vydání: 2006
Předmět:
Zdroj: Journal of Chemical Ecology. 32:1539-1554
ISSN: 1573-1561
0098-0331
DOI: 10.1007/s10886-006-9068-1
Popis: Females of the parasitic wasp Glyptapanteles flavicoxis (Hymenoptera: Braconidae) deposit a close-range sex pheromone from their abdominal tip that attracts conspecific males and elicits wing-fanning behavior. In this study, we isolated the pheromone components and determined their role in the males' behavior. In coupled gas chromatographic-electroantennographic detection (GC-EAD) analyses of the females' body extract, four components (below GC detection) elicited strong responses from male antennae. Monitored by GC-EAD, the components were separated by flash silica gel and high-performance liquid chromatography. Y-tube olfactometer experiments with one or more components revealed that all are necessary to elicit short-range attraction and wing-fanning responses by males. These components remained below detection threshold of the mass spectrometer (approximately 10 pg) even when 4500 female equivalents were analyzed in a single injection, which attests to the potency of the pheromone and the insects' sensitivity to it.
Databáze: OpenAIRE