Evidence for Four-Component Close-Range Sex Pheromone in the Parasitic Wasp Glyptapanteles flavicoxis
Autor: | Regine Gries, Paul W. Schaefer, Gerhard Gries, Adela Danci |
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Rok vydání: | 2006 |
Předmět: |
Male
Chromatography Gas biology Wasps Zoology General Medicine Hymenoptera biology.organism_classification Biochemistry Attraction Parasitoid Electroantennography Olfactometer Sex pheromone Botany Animals Pheromone Female Sex Attractants Braconidae Chromatography High Pressure Liquid Ecology Evolution Behavior and Systematics |
Zdroj: | Journal of Chemical Ecology. 32:1539-1554 |
ISSN: | 1573-1561 0098-0331 |
DOI: | 10.1007/s10886-006-9068-1 |
Popis: | Females of the parasitic wasp Glyptapanteles flavicoxis (Hymenoptera: Braconidae) deposit a close-range sex pheromone from their abdominal tip that attracts conspecific males and elicits wing-fanning behavior. In this study, we isolated the pheromone components and determined their role in the males' behavior. In coupled gas chromatographic-electroantennographic detection (GC-EAD) analyses of the females' body extract, four components (below GC detection) elicited strong responses from male antennae. Monitored by GC-EAD, the components were separated by flash silica gel and high-performance liquid chromatography. Y-tube olfactometer experiments with one or more components revealed that all are necessary to elicit short-range attraction and wing-fanning responses by males. These components remained below detection threshold of the mass spectrometer (approximately 10 pg) even when 4500 female equivalents were analyzed in a single injection, which attests to the potency of the pheromone and the insects' sensitivity to it. |
Databáze: | OpenAIRE |
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