RNU (Foxn1RNU-Nude) Rats Demonstrate an Improved Ability to Regenerate Muscle in a Volumetric Muscle Injury Compared to Sprague Dawley Rats
| Autor: | Zvi Schwartz, Shirley Zhang, Michael J. McClure, Tri Nguyen, Yen Chen Huang, Lucas C. Olson, David J. Cohen, Barbara D. Boyan |
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| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty muscle Foxn1 Cell decellularized tissue scaffold Bioengineering lcsh:Technology Article 03 medical and health sciences 0302 clinical medicine Immune system RNU medicine lcsh:QH301-705.5 Immunodeficiency 030304 developmental biology 0303 health sciences Decellularization lcsh:T business.industry CD68 Regeneration (biology) Skeletal muscle medicine.disease medicine.anatomical_structure lcsh:Biology (General) regeneration Immunohistochemistry nude rat business 030217 neurology & neurosurgery |
| Zdroj: | Bioengineering Volume 8 Issue 1 Bioengineering, Vol 8, Iss 12, p 12 (2021) |
| ISSN: | 2306-5354 |
| DOI: | 10.3390/bioengineering8010012 |
| Popis: | Products developed for skeletal muscle regeneration frequently incorporate allogeneic and xenogeneic materials to elicit a regenerative response to heal skeletal muscle wounds. To avoid graft rejection in preclinical studies, immunodeficient rodents are used. Whether the immunodeficiency alters the host response to the material in skeletal muscle has not been studied. In this study, we hypothesized that an allogeneic acellular skeletal muscle grafts implanted in an immunodeficient rat (RNU, Foxn1-deficient) would exhibit better new muscle fiber formation compared to grafts implanted in immunocompetent Sprague Dawley (SD) rats. Decellularized SD skeletal muscle matrix (DMM) was implanted in the gastrocnemius (N = 8 rats/group). 56 days after surgery, animal gait was examined and animals were euthanized. Muscle force was assessed and fiber number as well as immune cell infiltrate was measured by histomorphometry and immunohistochemistry. Animal gait and percent recovery of muscle force were unchanged in both groups, but newly regenerated muscle fibers increased in RNU rats. Macrophage staining for CD68 was higher in RNU rats than in SD rats. These data show differences in muscle regeneration between animal models using the same biomaterial treatment, but these differences could not be ascribed to the immune response. Overall, our data provide awareness that more studies are needed to understand how host responses to biomaterials differ based on the animal model used. |
| Databáze: | OpenAIRE |
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