Increased procoagulant cell-derived microparticles (C-MP) in splenectomized patients with ITP
| Autor: | Robert C. Duncan, Yeon-Soong Ahn, Eugene R. Ahn, Lawrence L. Horstman, Pamela Dudkiewicz, W. Jy, Vincenzo Fontana |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Erythrocytes medicine.medical_treatment Splenectomy Flow cytometry Blood cell immune system diseases Cell-Derived Microparticles Risk Factors hemic and lymphatic diseases medicine Humans Platelet Purpura Thrombocytopenic Idiopathic medicine.diagnostic_test Vascular disease business.industry Thrombosis Hematology Middle Aged medicine.disease Blood Coagulation Factors Red blood cell medicine.anatomical_structure Coagulation Cardiovascular Diseases Case-Control Studies Immunology Female business |
| Zdroj: | Thrombosis research. 122(5) |
| ISSN: | 0049-3848 |
| Popis: | Splenectomy is frequently employed for therapeutic and diagnostic purposes in various clinical disorders. However its long-term safety is not well elucidated. Although risk of infection by encapsulated organisms is widely recognized, less well-known are risks of thrombosis and cardiovascular disease.We investigated levels of cell-derived microparticles (C-MP) in 23 splenectomized ITP (ITP-S) and 53 unsplenectomized ITP patients (ITP-nS). Assay of C-MP derived from platelets (PMP), leukocytes (LMP), red cells (RMP) and endothelial cells (EMP) were performed by flow cytometry. Coagulation parameters included PT, aPTT and activities of FVIII, IX and XI. Results of all measures were compared between the two groups, ITP-S vs ITP-nS.Levels of all C-MP were higher in ITP-S than ITP-nS but only RMP and LMP reached statistical significance (p = 0.0035 and p0.0001, respectively). The aPTT was significantly shorter in ITP-S (p = 0.029). Interestingly, correlation analysis revealed that RMP, but not other C-MP, were associated with shortening of aPTT (p = 0.024) as well as with increased activities of factors VIII (p = 0.023), IX (p = 0.021) and XI (p = 0.0089).RMP and LMP were significantly elevated in splenectomized compared to non-splenectomized ITP patients. This suggests that the spleen functions to clear procoagulant C-MP, and that elevation of C-MP might contribute to increased risk of thrombosis, progression of atherosclerosis and cardiovascular disease following splenectomy. |
| Databáze: | OpenAIRE |
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