Polymorphism of the ovine calpastatin gene in some Turkish sheep breeds

Autor:	Nezih Ata, Yalçın Yaman, Ibrahim Cemal, Orhan Karaca, Onur Yilmaz, Tamer Sezenler
Rok vydání:	2014
Předmět:	Genetics education.field_of_study Veterinary medicine General Veterinary Population Biology Breed Genotype frequency Meat tenderness Calpastatin sheep PCR-RFLP MspI Genotype Allele education Allele frequency Calpastatin
Zdroj:	Volume: 38, Issue: 4 354-357 Turkish Journal of Veterinary and Animal Sciences
ISSN:	1303-6181 1300-0128
DOI:	10.3906/vet-1401-13
Popis:	The calpastatin (CAST) gene has a major effect on muscle growth and meat tenderness after slaughter; it is located on the fifth chromosome in sheep. Blood samples were collected from 720 animals in total from Kıvırcık (KIV), Sakız (SZ), Karacabey Merino (KM), and Gökçeada (GA) sheep populations raised in West Anatolia. The PCR products were digested by the restriction endonuclease MspI. Allele frequencies for M and N alleles of the gene were found to be 0.85 and 0.15 in KIV, 0.80 and 0.20 in KM, 0.99 and 0.01 in GA, and 0.34 and 0.66 in SZ sheep, respectively. It was determined that NN genotype frequency was quite lower in KIV (0.04) and KM (0.07) populations than in the SZ breed (0.40). On the other hand, the NN genotype was not observed in the GA population. Populations other than KM were found to be in Hardy–Weinberg equilibrium. In general, allele and genotype frequencies of CAST were similar to those in other studies on different sheep populations. This research is a beginning step for finding candidate genes’ effects on meat quantity and quality.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e7a0dad516700ce4edeed4e7b3a0f7a https://doi.org/10.3906/vet-1401-13 Zobrazit plný text záznamu Plný text ve formátu PDF