Isolation and characterization of phenanthrene-degrading Sphingomonas paucimobilis strain ZX4
| Autor: | Xue-jun Duan, Ying Xia, Yang-fang Ye, Ji Liu, Zhenmei Lv, Gang Rao, Hang Min |
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| Rok vydání: | 2005 |
| Předmět: |
DNA
Bacterial Sphingomonas paucimobilis Environmental Engineering Operon Molecular Sequence Data Bioengineering DNA Ribosomal Sphingomonas Microbiology Catechol 2 3-Dioxygenase Dioxygenases chemistry.chemical_compound Soil Pollutants Environmental Chemistry Amino Acid Sequence Gene Ribosomal DNA Phylogeny Soil Microbiology Glutathione Transferase Base Composition Base Sequence biology Proteins Phenanthrenes Phenanthrene biology.organism_classification 16S ribosomal RNA Pollution Biodegradation Environmental Biochemistry chemistry Genes Bacterial Bacteria Salicylic acid |
| Zdroj: | Biodegradation. 16:393-402 |
| ISSN: | 1572-9729 0923-9820 |
| DOI: | 10.1007/s10532-004-2412-7 |
| Popis: | Phenanthrene-degrading bacterium strain ZX4 was isolated from an oil-contaminated soil, and identified as Sphingomonaspaucimobilis based on 16S rDNA sequence, cellular fatty acid composition, mol% G + C and Biolog-GN tests. Besides phenanthrene, strain ZX4 could also utilize naphthalene, fluorene and other aromatic compounds. The growth on salicylic acid and catechol showed that the strain degraded phenanthrene via salicylate pathway, while the assay of catechol 2, 3-dioxygenase revealed catechol could be metabolized through meta-cleavage pathway. Three genes, including two of meta-cleavage operon genes and one of GST encoding gene were obtained. The order of genes arrangement was similar to S-type meta-pathway operons. The phylogenetic trees based on 16S rDNA sequence and meta-pathway gene both revealed that strain ZX4 is clustered with strains from genus Sphingomonas. |
| Databáze: | OpenAIRE |
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