Differentiation of K562 Cells Under ELF-EMF Applied at Different Time Courses
| Autor: | Inhan-Garip Ayşe, Tunaya Kalkan, Oncul Sule, Işal-Turgut Işil, Akan Zafer |
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| Rok vydání: | 2010 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species Time Factors animal structures Single exposure Reactive oxygen species metabolism Cellular differentiation Biophysics Medicine (miscellaneous) Cell Differentiation General Medicine respiratory system Cell biology Toxicology chemistry.chemical_compound Electromagnetic Fields chemistry Humans Magnesium Heat shock K562 Cells Reactive Oxygen Species Heat-Shock Response Hemin K562 cells |
| Zdroj: | Electromagnetic Biology and Medicine. 29:122-130 |
| ISSN: | 1536-8386 1536-8378 |
| Popis: | The time-course of ELF-EMF application to biological systems is thought to be an important parameter determining the physiological outcome. This study investigated the effect of ELF-EMF on the differentiation of K562 cells at different time courses. ELF-EMF (50 Hz, 5 mT, 1 h) was applied at two different time-courses; first at the onset of hemin induction for 1 h, and second, daily 1 h for four days. While single exposure to ELF-EMF resulted in a decrease in differentiation, ELF-EMF applied everyday for 1 h caused an increase in differentiation. The effect of co-stressors, magnesium, and heat-shock was also determined and similar results were obtained. ELF-EMF increased ROS levels in K562 cells not treated with hemin, however did not change ROS levels of hemin treated cells indicating that ROS was not the cause. Overall, these results imply that the time-course of application is an important parameter determining the physiological response of cells to ELF-EMF. |
| Databáze: | OpenAIRE |
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