Genomic characteristics of pancreatic squamous cell carcinoma, an investigation by using high throughput sequencing after in-solution hybrid capture
| Autor: | Junning Zhang, Wei-Ming Duan, Meng Shen, Jie Yu, Zeyi Liu, Fei-Ran Gong, Shu-Ling Liu, Liu-Mei Shou, Min Tao, Yi-Zhong Feng, Wei Li, Meng-Dan Xu, Meng-Yao Wu, Dong-Mei Gu, Kai Chen, Qiang Liu, Qi Zhu, Qiaoming Zhi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty in-solution hybrid capture Pancreatic squamous cell carcinoma Adenocarcinoma medicine.disease_cause Polymorphism Single Nucleotide Diagnosis Differential 03 medical and health sciences 0302 clinical medicine INDEL Mutation Pancreatic cancer Keratin pancreatic adenocarcinoma Biomarkers Tumor medicine pancreatic squamous cell carcinoma Humans chemistry.chemical_classification Mutation business.industry Tumor Suppressor Proteins Keratin-6 High-Throughput Nucleotide Sequencing Genomics medicine.disease Immunohistochemistry high throughput sequencing (HTS) Gene Expression Regulation Neoplastic Pancreatic Neoplasms Keratin 5 stomatognathic diseases Gene Ontology 030104 developmental biology medicine.anatomical_structure Oncology chemistry 030220 oncology & carcinogenesis Carcinoma Squamous Cell Keratin-5 Pancreas business Research Paper Transcription Factors |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.14678 |
| Popis: | Squamous cell carcinoma (SCC) of pancreas is a rare histotype of pancreatic ductal carcinoma which is distinct from pancreatic adenocarcinoma (AC). Although there are standard treatments for pancreatic AC, no precise therapies exist for pancreatic SCC. Here, we screened 1033 cases of pancreatic cancer and identified 2 cases of pure SCC, which were pathologically diagnosed on the basis of finding definite intercellular bridges and/or focal keratin peal formation in the tumor cells. Immunohistochemistry assay confirmed the positive expression of CK5/6 and p63 in pancreatic SCC. To verify the genomic characteristics of pancreatic SCC, we employed in-solution hybrid capture targeting 137 cancer-related genes accompanied by high throughput sequencing (HTS) to compare the different genetic variants in SCC and AC of pancreas. We compared the genetic alterations of known biomarkers of pancreatic adenocarcinoma in different pancreatic cancer tissues, and identified nine mutated genes in SCC of pancreas: C7orf70, DNHD1, KPRP, MDM4, MUC6, OR51Q1, PTPRD, TCF4, TET2, and nine genes (ABCB1, CSF1R, CYP2C18, FBXW7, ITPA, KIAA0748, SOD2, SULT1A2, ZNF142) that are mutated in pancreatic AC. This study may have taken one step forward on the discovery of potential biomarkers for the targeted treatment of SCC of the pancreas. |
| Databáze: | OpenAIRE |
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