| Autor: |
Joussef-Pi��a, Samira, Nankya, Immaculate, Nalukwago, Sophie, Baseke, Joy, Rwambuya, Sandra, Winner, Dane, Kyeyune, Fred, Chervenak, Keith, Thiel, Bonnie, Asaad, Robert, Dobrowolski, Curtis, Luttge, Benjamin, Lawley, Blair, Kityo, Cissy M., Boom, W. Henry, Karn, Jonathan, Qui��ones-Mateu, Miguel E. |
| Rok vydání: |
2022 |
| DOI: |
10.6084/m9.figshare.18482085 |
| Popis: |
Additional file 3: Figure S2. Sensitivity of EDITS primers. A Nested PCR primers nF6026 and nR6773 were evaluated using a serial dilution of DNA from the HIV-1 molecular clone pNL4-3 (0% to 100%) in a background of DNA from the non-HIV plasmid pUC19, at a final concentration of 0.1 ng/ml. DNA mixtures were amplified using Standard and Real-time PCR. Amplicons from the standard PCR were also deep sequenced and vpu/env mapped reads quantified using the DEEPGEN��� Software Tool Suite. Mean mapped reads and standard deviation are depicted. B ACH-2, a cell line latently infected with a copy of HIV-1 per cell, and MT-4, an HIV-negative human T cell line, were quantified and serial dilutions used to prepare seven mixtures containing 0 to 1000 CH-2 cells in a background of one million MT-4cells. Cell mixtures were ctivated with 100 ��g/ml of Concanavalin A and cell-associated spliced HIV-1 RNA quantified using EDITS as described in ���Methods��� section. Mean mapped reads and standard deviation are depicted. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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